Sign in →

Test Code JAK2 JAK2 V617F Mutation Screen


JAK2 V617F

Essential Thrombocythemia


Myeloproliferative Disorder


Polycythemia Vera


Sample Requirements

Specimen:  Whole Blood or Bone Marrow

Container(s):  Lavender/EDTA (preferred)

Preferred Vol:  6 mL blood; 1-2 mL bone marrow

Minimum Vol: 

Processing Instructions

Reject due to

Spin:  NO

Aliquot:  NO

Temp:  refrigerate; DO NOT FREEZE

Notes:  Bone marrow or peripheral blood: Ship at ambient temperature within 24 hours of collection.

Storage location:  Store sample in CPA refrigerator sendouts rack. 


Off-site collection: Send whole blood refrigerated. Optimal results are achieved when samples are received within: 72 hours of collection for bone marrow and peripheral blood






Specimen Type Temperature Time
Whole blood Room temp 48 h
Whole blood Refrigerated 7 d
Whole blood Frozen N
Extracted DNA Room temp 3-4 d
Extracted DNA Refrigerated 1 y
Extracted DNA Frozen Indefinitely



STAT Performed TAT
N Mon -Fri. 10 days


Performing Laboratory

UW Hematopathology



Department:  UW Hematopathology Laboratory, 825 Eastlake Ave E, Seattle, WA, 98195

Phone Number:  (206) 685-6066


Reference Range

Interpretive report provided.


Method:  PCR amplification of JAK2 from genomic DNA, specific for V617F point mutation

Analytical Volume:  None specified

Limitations:  None specified

CPT Codes

81270  (Updated by sstasi 07/12/2016)

Special Instructions

Links to:



Clinical Utility

The somatic point mutation V617F in the JAK2 tyrosine kinase gene (JAK2V617F) has been associated with several chronic myeloproliferative disorders, including polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF).

Send Out Instructions


Reference Test Name: JAK2 V617F DNA Screen
Reference Test Number: JAK2

Send on next available shuttle to UW Hematopathology Laboratory