Sign in →

Test Code JAK2 JAK2 V617F Mutation Screen

Synonyms

JAK2 V617F

Essential Thrombocythemia

Myelofibrosis

Myeloproliferative Disorder

Polycythemia

Polycythemia Vera

 

Sample Requirements

Specimen:  Whole Blood or Bone Marrow

Container(s):  Lavender/EDTA (preferred)

Preferred Vol:  6 mL blood; 1-2 mL bone marrow

Minimum Vol: 

Processing Instructions

Reject due to

Spin:  NO

Aliquot:  NO

Temp:  refrigerate; DO NOT FREEZE

Notes:  Bone marrow or peripheral blood: Ship at ambient temperature within 24 hours of collection.

Storage location:  Store sample in CPA refrigerator sendouts rack. 

 

Off-site collection: Send whole blood refrigerated. Optimal results are achieved when samples are received within: 72 hours of collection for bone marrow and peripheral blood

 

 

 

Stability

 

Specimen Type Temperature Time
Whole blood Room temp 48 h
Whole blood Refrigerated 7 d
Whole blood Frozen N
Extracted DNA Room temp 3-4 d
Extracted DNA Refrigerated 1 y
Extracted DNA Frozen Indefinitely

 

Availability

STAT Performed TAT
N Mon -Fri. 10 days

 

Performing Laboratory

UW Hematopathology

Department

 

Department:  UW Hematopathology Laboratory, 825 Eastlake Ave E, Seattle, WA, 98195

Phone Number:  (206) 685-6066

 

Reference Range

Interpretive report provided.

Methodology

Method:  PCR amplification of JAK2 from genomic DNA, specific for V617F point mutation

Analytical Volume:  None specified

Limitations:  None specified

CPT Codes

81270  (Updated by sstasi 07/12/2016)

Special Instructions

Links to:

 

UWMC

Clinical Utility

The somatic point mutation V617F in the JAK2 tyrosine kinase gene (JAK2V617F) has been associated with several chronic myeloproliferative disorders, including polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF).

Send Out Instructions

 

Reference Test Name: JAK2 V617F DNA Screen
Reference Test Number: JAK2
Instructions:

Send on next available shuttle to UW Hematopathology Laboratory