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Test Code KOH Exam KOH Exam

Biohazard Infectious

Clinical System Name

KOH Prep Only

Synonyms

Calcofluor Stain

Direct Fungal Exam

Fungal Prep

KOH Prep

 

Description

Includes KOH examination for the presence of yeast or fungal elements. This order does not include a culture for Fungus. See Fungal Culture, to request a full culture, including a KOH exam. 

 

Potasium Hydroxide (KOH) digests human tissue cells and helps reveal fungal elements. It is used primarily for hair, skin, and nail samples. For genital samples, yeast are easily seen on gram stain (see Gram Stain).

Sample Requirements

Specimen: Hair: Collect several affected hairs including the root, and scrapings from affected area on scalp.

Skin: Obtain skin scrapings from the leading edge of the lesion preferably using a scalpel blade, or alternatively the edge of a microscope slide.

Nails: Clip infected nail, or obtain scrapings from the affected area using a scalpel blade.

Container: Sterile Container

Preferred Vol: N/A

Minimum Vol: N/A

 

Processing Instructions

 

CPA: Send sample to Microbiology for processing.  If the sample is shared and only one tube, syringe or other container is received, affix shared sample label and send entire sample plus requisition to Microbiology.

 

Off-site collection: Off-site Children's Lab locations should send the sample to the SCH Microbiology Laboratory for processing. Sample should be kept at room temperature prior to and during courier transport. 

 

Other off-site locations, keep sample at room temperature prior to and during courier transport to the SCH Microbiology Laboratory. 

Stability

Temp Time
Room temp 2 hours
Refrigerated N
Frozen N

 

Availability

Procedure Performed Turn Around Time (TAT)
KOH 24/7

2 hours

 

 

Performing Laboratory

Seattle Children's Laboratory

 

Department

Microbiology Laboratory

CPT Codes

87220

 

This test is not limited to the CPT codes listed.  Others may be added as more testing is necessary and depending on the pathogens isolated (example: MIC's and ID codes)

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:

http://www.doh.wa.gov/PublicHealthandHealthcareProviders/NotifiableConditions.aspx

 

See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

http://www.doh.wa.gov/Portals/1/Documents/1200/phsd-LHJ.pdf

Reporting Information

Final Report: 24 hours