Sign in →

Test Code LACT Lactic Acid Blood

Important Note

NO MICROTAINERS. Read "Notes" under Sample Requirements before collection.

Place specimen in outside pocket of a pre-filled biohazard bag of ice, then put into a separate biohazard bag for transport.

Capillary blood gas tubes are not acceptable for off-site collection.

Clinical System Name

Lactic Acid Blood

Synonyms

Lactate

Lactate, Syringe

Lactic Acid, Syringe

Description

The concentration of lactate in the blood is dependent on the rate of production in muscle cells and erythrocytes and the rate of metabolism in the liver.  Lactic acidosis usually results from overproduction or underutilization of lactate.  Elevated lactate levels can occur as a result of tissue hypoxia; diabetes mellitus; phenformin therapy; malignancies; glycogen storage disease; ethanol, methanol or salicylate ingestion; and metabolic acidosis.      

Sample Requirements

Specimen:  Whole Blood

Container(s):  Heparinized Syringe, Dark Green/Sodium Heparin, Lt. Grey Sodium Fluoride, capillary blood gas tubes

Required Vol: 2 mL

Minimum Vol: 0.5mL (Syringe only) or 2 capillary blood gas tubes

 

Notes:

Place specimen in outside pocket of a pre-filled biohazard bag of ice, then put into a separate biohazard bag for transport.

 *For 4.0 mL Dark Green/Sodium Heparin tubes, less than 1/2 full will be rejected. Do not remove vacutainer top to fill tube.
* Remove air bubbles from Heparinized Syringe  immediately after collection.

For hemodilution patients, note the collection site. Method is affected by exposure to RBC's (longer than 15 minutes), drawing blood with stasis, and by oxygen depletion (exercise, hypoxia, crying). Do not use tourniquet for more than 30 seconds at a time and do not allow patient to flex or clench fist.

If syringe sample is drawn, sample volume can be shared with blood gas, ionized calcium, =lytes, and =glu. 

 

Note: Capillary blood gas tubes must be filled completely and mixed thoroughly to dissolve and disperse the heparin. Deliver to Lab immediately, avoid air bubbles.  Ideally, whole blood specimens should be analyzed within 15 minutes of blood draw.

 

Processing Instructions

Reject due to:  Green Top or Gray Top not received on ice, age of specimen, wrong specimen container, hemolysis

Spin: N

Aliquot: N

Temp: 2-8oC

Storage location: Core Chemistry Refrigerator

 

Off-site collection: READ "COMMENTS/NOTES" BEFORE COLLECTION!* Do not use a tourniquet for more than 30 seconds at a time and do not have patient clench fist. Avoid hemolysis. PROCESS IMMEDIATELY. Spin blood within 15 minutes of draw, remove plasma and freeze in a plastic tube at -20°C. Ship on dry ice. Plasma must be removed from red blood cells within 15 minutes for an accurate lactic acid level.

*Capillary blood gas tubes are not acceptable for offsite collection.

Stability

Specimen Type Temperature Time
Whole Blood (Syringe or capillary) RT < 15 M
Whole Blood (Syringe or capillary)  0-4oC

30 min

Plasma* RT

8 H

Plasma* 2-8oC

14 D

Plasma* -20o C or -70oC

30 D

 

*For sample collected in a Green Top or Gray Top plasma must be removed from red blood cells within 15 minutes for an accurate lactic acid level.

Availability

STAT Performed TAT
Y 24/7 1 H

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Rapidpoint/Chemistry

 

 

 

Reference Range

0.5 - 2.2 mMol/L

Methodology

Whole blood Method: Amperometric by Siemens Rapid Point 500

Analytical Volume: 0.2mL

 

Plasma Method:  Colorimetric by Vitros 4600

Analytical Volume:  0.02 mL plasma + 0.03 mL dead space

CPT Codes

83605