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Test Code LD Lactate Dehydrogenase

Important Note

This test cannot be ordered as an add-on once it has been stored in fridge.

Clinical System Name

Lactate Dehydrogenase

Synonyms

LDH - Blood

Description

Causes of high lactate dehydrogenase include neoplastic states, hypoxic cardio respiratory diseases, myocardial infarctions, hemolytic anemias, megaloblastic anemias, hepatic cirrhosis, renal infarction, trauma, muscle damage muscular dystrophy, shock, and hypotension.  In myocardial infarction cases, LDH begins to rise within about 12 hours after infarction and usually returns to baseline after 2 to 5 days.

Sample Requirements

Specimen: Whole blood

Container(s): Gold SST, Red Tube, or Dark Green/Sodium Heparin,  Lt. Green/Lithium Heparin Microtainer, or Gold Microtainer

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL

 

Note:  Method is affected by oxalate, hemolysis and by exposure to RBC's. A slow freeze and thaw can result in a loss of up to 70% of LDH activity. Not affected by lipemia.

 

Processing Instructions

Reject due to: Age of specimen, incorrect collection container

Spin: Y

Aliquot: Y

Temp: 2-8oC

Storage location: Core 5 Refrigerator

 

Off-site collection:  Avoid hemolysis. Spin blood, separate serum/plasma from cells within one hour of collection. Transport specimen at room temperature. Do not refrigerate or freeze. Samples are stable for 2 days at room temperature.

Stability

 

Specimen Type Temperature Time
Serum or Plasma RT

2 D

Serum or Plasma 2-8oC

Not Recommended  

Serum or Plasma -20o C or -70oC

Not Recommended

 

Availability

STAT Performed TAT
Y 24/7 1 H

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Chemistry

Phone Number: 206-987-2617

 

 

Reference Range

 

  IU/L
0- 1 W Up to 2150
1 W- 5 Y 425 - 975
5 - 12 Y 370 - 840
12 - 14 Y 370 - 785
14 - 16 Y 370 - 645
>16 Y 313 - 618

 

Methodology

Method: Multiple Point Rate by Vitros 4600

Analytical Volume:  0.02 mL serum or plasma + 0.03 mL dead space

CPT Codes

83615