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Test Code LUPUS Lupus Inhibitor Assay

Clinical System Name

Lupus Inhibitor Assay
 

Synonyms

Lupus anticoagulant
Lupus inhibitor
Lupus-like Inhibitor Assay
Staclot-LA

Description

Lupus anticoagulants (LA) are immunoglobulins that generally interfere with phospholipid-dependent in vitro coagulation tests.LA is often seen in persons with no underlying diseases and no history of abnormal bleeding. LA also occurs in patients with autoimmune disease or as a reaction to infections or drugs. LA is also associated with thrombosis, including deep venous thrombosis (DVT) and pulmonary emboli (PE), arterial occlusion, pregnancy loss, and microvascular thrombi.

Sample Requirements

Specimen: Whole Blood

Container(s): Lt Blue/Citrate

Preferred Vol: (3) 1.8 mL or (1) 2.7 mL

Minimum Vol: (2) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the  collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

Specimen should be centrifuged within 1 hour of collection.

 

Processing Instructions

Reject due to: Clotted, Hemolyzed, Insufficient quantity, and  Improper collection.

Spin:N

Aliquot: N

Temp:RT

Storage location: Core 14 Freezer  ( -70 C).

 

Note: Deliver blood to Core Coag bench.  Coag Technologist will process. Specimen should be centrifuged within 1 hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label.  Freeze 2 aliquots of 1.0 mL plasma in plastic tubes at -70C.

 

 

 

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.

Specimen should be centrifuged within 1 hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze 2 aliquots of 1.0 mL plasma in plastic tubes at -70C.

Ship frozen on dry ice.

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen   -20 C or -70 C 3 m

 

Availability

STAT Performed TAT
N

THURSDAY

1 w

 

Performing Laboratory

Seattle Children's Laboratory    

 

Department

Department:  Core Lab/Coagulation

 

 

 

 

Reference Range

Negative

Methodology

Method: Electromagnetic mechanical clot detection system  by STA® - Compact Analyzer

Analytical Volume: 0.7 mL plasma.

 

Limitations: Patient should not be on anticoagulants,  may affect test results.

Method is affected by improper collection, processing and storage.

High hematocrit greater than or equal to 56% requires a citrate adjusted tube.

 

CPT Codes

85613

Critical Values

Not defined for this test.