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Test Code Lyme Ab WB Lyme Disease Antibody, Immunoblot, Serum

Reporting Name

Lyme Disease Ab, Immunoblot, S
Seattle Children's Hospital Note:

Clinical System Name: Lyme Disease Ab, Western Blot

Useful For

Diagnosing Lyme disease


IgM assay is useful for confirming stage 1 (acute) Lyme disease.


IgG assay is useful for confirming stage 2 and stage 3 Lyme disease.

Specimen Required


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL

Seattle Children's Hospital Note:

Collect 1.0 mL whole blood in a Red or Gold top.

Specimen Type


Specimen Minimum Volume

0.2 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Day(s) and Time(s) Performed

Monday through Friday; 9 a.m.

Saturday, Sunday; varies

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name


Method Description

In the immunoblot analysis, an antigen mixture prepared from Borrelia burgdorferi strain B31 is separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). After the antigens have been resolved by SDS-PAGE, they are electrophoretically transferred and bound to a nitrocellulose membrane. Patient specimen is then added to the nitrocellulose membrane. If Lyme-specific antibodies are present in the specimen, they will bind to antigen on the nitrocellulose membrane. Bands are then detected by the addition of an enzyme-labeled antihuman-IgG or antihuman-IgM reagent that allows for the visualization of bands on the nitrocellulose membrane.(Towbin H, Gordon J: Immunoblotting and dot immunobinding--current status and outlook. J Immunol Methods:1984;72:313-340; Johnson CM, Helgeson SC: Glycoproteins synthesized by cultured cardiac valve endothelial cells: unique absence of fibronectin production. Biochem Biophys Res Comm 1988;153:46-50; Grodzicki RL, Steere AC: Comparison of immunoblotting and indirect enzyme-linked immunosorbent assay using different antigen preparations for diagnosing early Lyme disease. J Infect Dis 1988;157:790-797)

Reference Values

IgG: negative

IgM: negative

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86617 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LYWB Lyme Disease Ab, Immunoblot, S Unable to Verify


Result ID Test Result Name Result LOINC Value
5744 IgG Immunoblot 6320-6
2992 IgG Band(s) 13502-0
23931 IgM Immunoblot 6321-4
23932 IgM Band(s) 13503-8
6241 Interpretation 12781-1

Reject Due To


Mild OK; Gross reject


Mild OK; Gross reject


Mild OK; Gross reject


Heat-inactivated specimen

Testing Algorithm

See Acute Tick-Borne Disease Testing Algorithm in Special Instructions.


If not ordering electronically, complete, print, and send a General Request Form (T239) with the specimen (