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Test Code MANGANESE Manganese, Serum

Reporting Name

Manganese, S

Useful For

Monitoring manganese exposure

 

Nutritional monitoring

 

Clinical trials


Specimen Required


Collection Container/Tube: Plain, royal blue-top Vacutainer plastic trace element blood collection tube (T184)

Submission Container/Tube: 7-mL Mayo metal-free, screw-capped, polypropylene vial (T173)

Specimen Volume: 1.6 mL

Collection Instructions:

1. Allow the specimen to clot for 30 minutes, and then centrifuge to separate serum from the cellular fraction. Serum must be removed from cellular fraction within 4 hours of draw. Avoid hemolysis.

2. Remove the stopper. Carefully pour specimen into a Mayo metal-free, polypropylene vial, while avoiding transfer of the cellular components of blood. Do not insert a pipet into the serum to accomplish transfer, and do not ream the specimen with a wooden stick to assist with serum transfer.

3. See Trace Metals Analysis Specimen Collection and Transport in Special Instructions for complete instructions.

Additional Information:

1. High concentrations of gadolinium and iodine are known to interfere with most metals tests. If either gadolinium- or iodine-containing contrast media has been administered, a specimen should not be collected for 96 hours.

2. If ordering the trace element blood collection tube from BD, order catalog #368380.


Seattle Children's Hospital Note:

Collect 3.2 mL whole blood in a Royal Blue top trace element tube.

 

Processing Note: Use of a standard pipette is acceptable for aliquoting these samples.

Specimen Type

Serum

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  7 days

Day(s) and Time(s) Performed

Tuesday, Friday; 8 a.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Dynamic Reaction Cell-Inductively Coupled Plasma-Mass Spectrometry (DRC-ICP-MS)

Method Description

This assay is performed on an inductively coupled plasma-mass spectrometer in dynamic reaction cell mode. Calibrating standards and blanks are diluted with an aqueous acidic diluent containing internal standards. Quality control specimens and patient samples are diluted in an identical manner. In turn, all diluted blanks, calibrating standards, quality control specimens, and patient specimens are aspirated into a pneumatic nebulizer and the resulting aerosol directed to the hot plasma discharge by a flow of argon. In the annular plasma the aerosol is vaporized, atomized, then ionized. The ionized gases, plus neutral species formed in the annular plasma space, are aspirated from the plasma through an orifice into a quadrupole mass spectrometer. The mass range from 1 amu to 263 amu is rapidly scanned multiple times and ion counts tabulated for each mass of interest. Instrument response is defined by the linear relationship of analyte concentration vs. ion count ratio (analyte ion count/internal standard ion count). Analyte concentrations are derived by reading the ion count ratio for each mass of interest and determining the concentration from the response line.(Unpublished Mayo method)

Reference Values

<2.4 ng/mL

Reference values have not been established for patients that are <18 years of age.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

83785

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MNS Manganese, S 5683-8

 

Result ID Test Result Name Result LOINC Value
8413 Manganese, S 5683-8

Reject Due To

Hemolysis

Mild reject; Gross reject

Lipemia

Mild OK; Gross OK

Icterus

Mild OK; Gross reject

Other

NA