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Test Code MISC OncoPlex

Synonyms

OncoPlex Cancer Gene Panel

OPX

Description

UW-OncoPlex is a multiplexed mutation assay for tumor tissue that assesses mutations in 262 genes related to cancer treatment, prognosis, or diagnosis. The genes are sequenced on an Illumina instrument to detect single nucleotide variants, insertions, deletions, gene amplifications, and selected translocations. Microsatellite instability (MSI) status is reported for colorectal cancer cases. This test is designed to detect somatic mutations in cancer, and is not designed to detect germline (heritable) mutations.

Sample Requirements

Specimen: Whole blood, Bone Marrow, or Purified DNA

Container(s): Lavender/EDTA

Preferred Vol: 6 mL

Minimum Vol: 3mL

 

Specimen: Tissue samples:  Send EITHER (a) tissue block, OR (b) curls, OR (c) slides:

Container(s):

Preferred Vol:

Minimum Vol:

 

 

Note: If collecting bone marrow, the sample must be drawn in a Lavender/EDTA tube. Various tissue samples are also acceptable. For more information, see UW Medicine LabMed link at the bottom of this page.

 

Unacceptable:  Decalcified samples; tissue samples treated with fixatives other than formalin.

Processing Instructions

Reject due to: Frozen or clotted specimens

Spin: N

Aliquot: N

Temp: Refrigerated

Storage location: Affix large Cerner label(s) to tube(s) and place in refrigerated Send Outs rack. Store extra labels and a copy of requisition in the clear plastic box attached to CPA refrigerator. Alert Send Outs team.

 

Off-site collection: Send whole blood refrigerated

Stability

Specimen Type Temperature Time
Whole Blood Room temp 3 d
Whole Blood Refrigerated 7 d
Whole Blood Frozen

N

 

Availability

STAT Performed TAT
No   4 - 6 w

 

Performing Laboratory

University of Washington Medical Center

Department

UW Medical Center

Laboratory Medicine – Genetics Lab

1959 NE Pacific St, Room NW220

SEATTLE, WA 98195-7110

 

Phone: (206) 598-6429

Methodology

Method: UW-OncoPlex is a multiplexed gene sequencing panel that detects mutations in tumor tissue in 262 cancer-related genes (listed in the methods below). The panel includes genes related to cancer treatment, prognosis, and diagnosis. The test uses next-generation "deep" sequencing to detect most classes of mutations, including single nucleotide variants, small insertions and deletions (indels), gene amplifications, and selected gene-fusions. In addition, microsatellite instability status is reported for colorectal cancers.

Analytical Volume: 3 mL for Whole Blood or Bone Marrow, 3 mcg of Purified DNA

Limitations:

Send Out Instructions

Reference Test Name UW OncoPlex Cancer Gene Panel
Reference Test Number OPX
Instructions Ship refrigerated. Avoid freezing the sample. Send out M thru F with the UW courier. Keep a copy of the Batch Sheet for our records.

 

UWMC Genetics Lab page:

http://depts.washington.edu/labweb/Divisions/MolDiag/MolDiagGen/index.htm

 

UWMC Genetics Lab requisition:

http://depts.washington.edu/labweb/referencelab/clinical/TestForms/genetics.pdf

Special Instructions

Links to:

 

University of Washington