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Test Code MPL UM MPL Codon 515 Mutation Screen  

Clinical System Name

MPL Codon 515 Mutation Screen  


W515K, W515L

Essential thrombocythemia

Myeloproliferative neoplasms

Primary myelofibrosis


Somatic mutations in the MPL gene have been infrequently identified in chronic myeloproliferative neoplasms, including JAK2 mutation-negative and CALR mutation-negative essential thrombocythemia (ET) and primary myelofibrosis (PMF).

Sample Requirements


Blood: 6 ml blood in LAVENDER TOP (EDTA) tubes OR

Bone Marrow: 1-2 mL BONE MARROW anticoagulated with EDTA



Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temp: 2-8 C

Storage location: Affix large Cerner labels to tubes and place in refrigerator send-outs rack.


Off-site collection: Send whole blood refrigerated. Optimal results are achieved when samples are received within: 72 hours of collection for bone marrow and peripheral blood


Specimen Type Temperature Time
Whole blood Room temp 48 h
Whole blood Refrigerated 7 d
Whole blood Frozen N
Extracted DNA Room temp 3-4 d
Extracted DNA Refrigerated 1 y
Extracted DNA Frozen Indefinitely




STAT Performed TAT
N Accepted Mon - Fri from 0800 - 1500.  Performed as needed Results within 7 working days


Performing Laboratory

UW Hematopathology



Department:  UW Hematopathology Laboratory, 825 Eastlake Ave E, Seattle, WA, 98195

Phone Number:  (206) 685-6066


Reference Range

Interpretive report is provided.


Method: Allele specific PCR followed by capillary electrophoresis. Detects the W515L or

the W515K mutation, but does not test for the S505N mutation.

Analytical Volume: None specified

Limitations: None specified

CPT Codes

81402                (updated 2/4/2016 by sstasi) 

Clinical Utility

Candidates for this test are patients with a suspicion for myeloproliferative neoplasms (MPN) in JAK2 V617F and CALR exon 9 negative individuals.

Send Out Instructions

Reference Test Name: MPL Codon 515 Mutation Screen
Reference Test Number: MPLAS
Instructions: Send on next available shuttle to UW Hematopathology Laboratory