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Test Code MYCOPL AB Mycoplasma pneumoniae Antibodies, IgG and IgM, Serum


Advisory Information


Detection of IgM or IgG class antibodies to Mycoplasma pneumoniae provides exposure information. The preferred method of diagnosis of acute M pneumoniae infection is by molecular detection: MPRP / Mycoplasma pneumoniae, Molecular Detection, PCR.



Specimen Required


Collection Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL


Useful For

Screen for recent or past exposure to Mycoplasma pneumoniae

Profile Information

Test ID Reporting Name Available Separately Always Performed
MYCOG M. pneumoniae Ab, IgG, S No Yes
MYCOM M. pneumoniae Ab, IgM, S No Yes
MYCON M. pneumoniae Ab Interpretation No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
MMYCO M. pneumoniae Ab, IgM, S by IFA No No

Testing Algorithm

If Mycoplasma pneumoniae antibodies, IgM is positive or equivocal, then M pneumoniae antibodies, IgM by IFA will be performed at an additional charge.

Method Name

MYCOG, MYCOM: Enzyme Immunoassay (EIA)

MMYCO: Indirect Immunofluorescence Assay (IFA)

Reporting Name

M. pneumoniae Ab, IgG and IgM, S

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Hemolysis

Mild reject; Gross reject

Lipemia

Mild reject; Gross reject

Icterus

NA

Other

NA

Reference Values

IgG: Negative

IgM: Negative

IgM by IFA: Negative

Method Description

IgG:

Diluted sera are incubated in antigen-coated microwells. Any antigen-specific antibody in the samples will bind to the immobilized antigen. The plate is washed to remove unbound antibody and other serum components. Peroxidase conjugated goat-antihuman IgG is added to the wells and incubated. The conjugate will react with the IgG antibody/antigen on the solid phase. The wells are washed to remove unreacted conjugate. The microwells containing immobilized conjugate are incubated with peroxidase substrate solution. Hydrolysis of the substrate by peroxidase produces a color change. After a period of time the reaction is stopped by the addition of diluted acid, and the color changes are measured photometrically. The color intensity of the solution depends on the antibody concentration in the serum sample.(Package insert: Mycoplasma IgG ELISA II. Wampole Laboratories, Princeton, NJ 2004)

 

IgM EIA:

Test sera are diluted with the sample diluent provided. The sample diluent contains antihuman IgG that precipitates and removes IgG and rheumatoid factor from the sample, leaving IgM free to react with immobilized antigen. Diluted sera are incubated in antigen-coated microwells. Any antigen-specific antibody in the samples will bind to the immobilized antigen. The plate is washed to remove unbound antibody and other serum components. Peroxidase conjugated goat-antihuman IgM (chain specific) is added to the wells and incubated. The conjugate will react with the IgM antibody/antigen on the solid phase. The wells are washed to remove unbound conjugate. The microwells containing immobilized conjugate are incubated with peroxidase substrate solution. Hydrolysis of the substrate by peroxidase produces a color change. After a period of time the reaction is stopped by the addition of diluted acid, and the color changes are measured photometrically. The color intensity of the solution depends on the antibody concentration in the serum sample.(Package insert: Mycoplasma IgM ELISA II. Wampole Laboratories, Princeton, NJ 2004)

 

IgM Immunofluorescence Assay (IFA):

Mycoplasma pneumoniae antigenic substrate is fixed onto microscope slide wells. Serum that has been pretreated to remove IgG antibodies is incubated with the substrate. If IgM antibody to M pneumoniae is present, it will bind to the substrate. Fluorescein-labeled anti-human IgM conjugate is added to the slide wells and the slide is incubated. If antibody is present, it can be observed as a characteristic positive, bright, apple-green fluorescent reaction when the slide is read on a fluorescence microscope.(Package insert: Mycoplasma pneumoniae IgM IFA Antibody Test System. Zeus Scientific, Inc., Raritan, NJ 2004)

Day(s) and Time(s) Performed

Monday through Friday; 9 a.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

86738 x 2-Mycoplasma pneumoniae by EIA

86738-Mycoplasma pneumoniae by indirect IFA (if appropriate)

LOINC Code Information

Result ID Test Result Name Result LOINC Value
MYCOG M. pneumoniae Ab, IgG, S 45224-3
MYCOM M. pneumoniae Ab, IgM, S 5257-1
MYCON M. pneumoniae Ab Interpretation 69048-7