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Test Code Macroproltn Macroprolactin, Serum

Reporting Name

Macroprolactin, S

Useful For

Determining biologically active levels of prolactin, in asymptomatic patients with elevated prolactin levels

 

Ruling out the presence of macroprolactin


Specimen Required


Container/Tube: 

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 1 mL

Collection Instructions: Spin down and separate serum from clot.

Additional Information: Twelve hours before this blood test, do not take multivitamins or dietary supplements containing biotin or vitamin B7 that are commonly found in hair, skin and nail supplements and multivitamins.


Seattle Children's Hospital Note:

Collect 2.0 mL whole blood in a Red top.

Specimen Type

Serum

Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  180 days

Day(s) and Time(s) Performed

Monday through Friday 5 a.m. – 12 a.m., Saturday 6 a.m. – 6 p.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

TOPRL: Electrochemiluminescent Immunoassay

PEGPR: Polyethylene Glycol (PEG) Precipitation Followed by Electrochemiluminescent Immunoassay

Method Description

The instrument use for testing is the Roche Cobas 6000. An equal volume of 25% polyethylene glycol (PEG) is added to a serum prolactin sample causing the biologically inactive form of prolactin (macroprolactin) to precipitate. Following centrifugation, prolactin is measured in the supernatant (containing unprecipitated prolactin), as well as in an aliquot of the native sample. Precipitated prolactin is calculated by subtracting the unprecipitated prolactin value from the total prolactin. The percentage of precipitated prolactin to total prolactin indicated the presence or absence of macroprolactin.(Package insert: Elecsys and Modular Prolactin II, Roche 2005)

Reference Values

TOTAL PROLACTIN

Males

≤18 years: not established

>18 years: 4.0-15.2 ng/mL

Females

≤18 years: not established

>18 years: 4.8-23.3 ng/mL

 

Percent of the precipitated (complexed) prolactin fraction of the total prolactin <50% (considered negative for macroprolactin)

 

Unprecipitated prolactin levels are expected to be within the total prolactin reference range.

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

84146 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MCRPL Macroprolactin, S In Process

 

Result ID Test Result Name Result LOINC Value
PROU Prolactin,Unprecipitated,S 38926-2
T_PRL Prolactin,Total,S 2842-3
RATIO Percent 51441-4
CMT42 Comment 48767-8

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross OK

Icterus

NA

Other

NA

Profile Information

Test ID Reporting Name Available Separately Always Performed
TOPRL Prolactin,Total,S Yes, (order PRL) Yes
PEGPR Prolactin,Unprecipitated,S No Yes