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Test Code Mumps IgM Mumps Virus Antibody, IgM, Serum

Important Note

Ordering this test seeks evidence of acute infection. If so, the patient is contagious and Infection Control must be notified. Priority of testing (stat vs. routine) is determined by State Epidemiologist on call (206) 418-5500. Ordering provider must contact State Epidemiologist and then notify Lab of testing priority. If recommended by the State Epidemiologist, urine and throat swabs may be sent along with the serology to Washington State Laboratory. For general questions regarding this test, contact Dr. Xuan Qin x72586.

Reporting Name

Mumps Ab, IgM, S

Useful For

Laboratory diagnosis of mumps virus infection

Seattle Children's Hospital Note:

This test is exclusively for the purpose of ruling out an active Mumps infection.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 1 mL


Seattle Children's Hospital Note:

Collect 3 Gold SST Microtainers.

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reference Values

Negative (reported as positive, equivocal, or negative)

Index value 0.00-0.79=negative

Day(s) and Time(s) Performed

Monday through Friday; 4 p.m.

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86735

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MMPM Mumps Ab, IgM, S In Process

 

Result ID Test Result Name Result LOINC Value
MUMP1 Mumps Ab, IgM, S 6478-2
DEXM Index Value 25419-3

Method Description

The SeraQuest mumps IgM is an enzyme capture method. Diluted samples are incubated in wells coated with antihuman-IgM monoclonal antibodies. If present, IgM antibodies are captured in the wells. Wells are washed, removing excess sample. Conjugate-antigen complex (mumps antigen in a complex with monoclonal antibodies conjugated to horseradish peroxidase) is added and wells are incubated. IgM antibodies specific for the antigen will bind the conjugate. Wells are washed, removing excess conjugate. Peroxidase substrate is added and wells are incubated. Stop solution is added converting the substrate to a yellow end product which is read photometrically.(Package insert: Mumps IgM, SeraQuest, 1938 NE 148th Terrace, North Miami, FL 33181)

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

NA

Other

Heat-inactivated specimen

Method Name

Enzyme Immunoassay (EIA)