Sign in →

Test Code NSE SERUM Neuron-Specific Enolase (NSE), Serum

Reporting Name

Neuron Specific Enolase, S

Useful For

A follow-up marker in patients with neuron-specific enolase-secreting tumors of any type

 

An auxiliary test in the diagnosis of small cell lung carcinoma

 

An auxiliary test in the diagnosis of carcinoids, islet cell tumors and neuroblastomas

 

An auxiliary tool in the assessment of comatose patients

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic screw-top aliquot tube

Specimen Volume: 0.5 mL


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 7 days
  Ambient  7 days

Reference Values

≤15 ng/mL

Serum markers are not specific for malignancy, and values may vary by method.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

83520

LOINC Code Information

Test ID Test Order Name Order LOINC Value
NSE Neuron Specific Enolase, S 15060-7

 

Result ID Test Result Name Result LOINC Value
NSE Neuron Specific Enolase, S 15060-7

Reject Due To

Hemolysis

Mild reject; Gross reject

Lipemia

Mild OK; Gross OK

Icterus

Mild reject; Gross reject

Other

NA

Day(s) and Time(s) Performed

Monday through Saturday; 2:30 p.m.

Method Description

Neuron specific enolase (NSE) is measured in this homogeneous automated immunofluorescent assay on the BRAHMS Kryptor. The Kryptor uses TRACE (Time Resolved Amplified Cryptate Emission) technology based on a non-radioactive transfer of energy. This transfer occurs between 2 fluorescent tracers: the donor (europium cryptate) and the acceptor (XL665). In the NSE assay, 2 monoclonal antibodies are labeled, 1 with europium cryptate and 1 with XL665. NSE is sandwiched between the 2 antibodies, bringing them into close proximity. When the antigen-antibody complex is excited with a nitrogen laser at 337 nm, some fluorescent energy is emitted at 620 nm and the rest is transferred to XL665. This energy is then emitted as fluorescence at 665 nm. A ratio of the energy emitted at 665 nm to that emitted at 620 nm (internal reference) is calculated for each sample. Signal intensity is proportional to the number of antigen-antibody complexes formed, and therefore to antigen concentration.(Package insert: BRAHMS NSE Kryptor. Thermo Fisher Scientific BRAHMS LLC, Middletown, VA)

Method Name

Homogeneous Time-Resolved Fluorescence