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Test Code Neut Ox Bu Neutrophil Oxidative Burst

Important Note

Note:  Limited specimen stability - see Sample Requirements below.

Off-site Collection:  If specimen will not arrive at Children's before noon for same day testing, blood should be drawn as late in the day as possible, maintained at room temperature and sent overnight to allow test set-up the following morning.  See additional notes under 'Processing Instructions - Offsite Collection'.

Clinical System Name

Neutrophil Oxidative Burst

Synonyms

Metabolic Burst Assay
Respiratory Burst Assay

Description

This test is a flow cytometric assay of neutrophil function utilizing the dye dihydrorhodamine (DHR). The assay is used to aid in the diagnosis of Chronic Granulomatous Disease (CGD); x-linked carrier states can also be distinguished. Note:  The Nitroblue Tetrazolium Reduction (NBT) test is no longer available.

Sample Requirements

Specimen:  Whole Blood

Container(s):  Dark Green/Sodium Heparin (no serum separator);

Preferred Vol:  1 mL

Minimum Vol:  0.5 mL; no microtainers accepted

 

  Collected in-house at Seattle Children's Collected off-site (including SCH Bellevue)
M-Th collect any time collect any time
Friday collect any time ok if received at SCH main lab same day, if not do not collect
Saturday ok if collected before 12 noon do not collect
day before a holiday ok if collected before 12 noon must be received at SCH main lab before 12 noon
Sunday ok if collected after 12 noon (to be tested next morning)

ok if collected after 12 noon (to be tested next morning)

Sunday before a holiday Monday do not collect do not collect
Holiday ok if collected after 12 noon (to be tested next morning) ok if collected after 12 noon (to be tested next morning)

 

        ok if collected before 12 noon

 

Processing Instructions

Reject due to:  n/a - send to lab

Spin:  N

Aliquot:  N

Temp:  RT

Storage location:  Days:  Transport specimen, (copy of community services requisition, if applicable), and labels to 10th floor Cell Markers (station #280).  Notify Cell Markers at x72560 right away if specimen is near 20 hour specimen stability limit - billing/ordering problems should not delay delivery to lab.   Eves/Nights:  Store specimen, (copy of community services requisition, if applicable), and labels in the Cell Markers RT box in CPA.

 

Off-site collection:  A normal control should accompany the specimen. It is critical that samples be kept at room temperature; use extra packing to maintain temperature. If specimen will not arrive at Children's before noon for same day testing, blood should be drawn as late in the day as possible, maintained at room temperature and sent overnight. It is recommended that samples arrive in the lab within 20-24 hours of draw, however samples may be stable for up to 50 hours.  Transport specimens to Seattle Children's Hospital address on requisition: Laboratory OC.8.720, 4800 Sand Point Way NE, Seattle, WA  98105. 

Stability

Temperature Time
Room temp 20 hours recommended, may be stable to 50 hours, see Processing/Off-site Instructions
Refrigerated N
Frozen N

 

Availability

STAT Performed TAT
N

Monday - Friday

24 hours

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Cell Markers

Phone Number: 206-987-2560

 

 

Reference Range

Normal Stimulation Index >20

 

Methodology

Method:  Flow Cytometry

Analytical Volume:  0.5 mL whole blood

Limitations:  None specified

CPT Codes

CPT CODES

88184, 88185