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Test Code OLIGO BAND Oligoclonal Banding, Serum and Spinal Fluid

Reporting Name

Oligoclonal Banding

Useful For

Diagnosis of multiple sclerosis; especially useful in patients with equivocal clinical presentation and radiological findings


Specimen Required


Serum and spinal fluid are required. Spinal fluid must be obtained within 1 week of serum draw.

 

Specimen Type: Serum

Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 0.5 mL

Collection Instructions: Label specimen as serum.

 

Specimen Type: Spinal fluid

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Collection Instructions: Label specimen as spinal fluid.


Seattle Children's Hospital Note:

Collect 1.0 mL whole blood in a Red top.

Specimen Type

CSF
Serum

Specimen Minimum Volume

Serum: 0.4 mL/Spinal Fluid: 0.4 mL

Specimen Stability Information

Specimen Type Temperature Time
CSF Refrigerated (preferred) 14 days
  Ambient  14 days
  Frozen  14 days
Serum Refrigerated (preferred) 14 days
  Ambient  14 days
  Frozen  14 days

Day(s) and Time(s) Performed

Monday through Saturday; 7 a.m.-12 p.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Isoelectric Focusing (IEF) with IgG Immunoblot Detection

Method Description

The oligoclonal band (OCB) assay requires paired cerebrospinal fluid (CSF) and serum samples. Unconcentrated CSF and diluted serum are electrophoresed by isoelectric focusing. The separated immunoglobulin (Ig) are visualized by an IgG immunoblot, and OCBs that are present in the CSF and not in the serum are reported. The assay uses reagents from Helena Laboratories. (Keir G, Luxton RW, Thompson EJ: Isoelectric focusing of cerebrospinal fluid immunoglobulins G: an annotated update. Ann Clin Biochem 1990;27:436-443)

Reference Values

CSF Olig Bands Interpretation: <4 bands

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

83916-Spinal fluid

83916-Serum

LOINC Code Information

Test ID Test Order Name Order LOINC Value
OLIG Oligoclonal Banding In Process

 

Result ID Test Result Name Result LOINC Value
8017 CSF Bands 56764-4
2783 Serum Bands 56974-9
23611 CSF Olig Bands Interpretation 49852-7

Reject Due To

Hemolysis

Mild OK; Gross OK

Lipemia

Mild OK; Gross OK

Icterus

Mild OK; Gross OK

Other

NA

Profile Information

Test ID Reporting Name Available Separately Always Performed
OLIGS Serum Bands No Yes
OLIGC CSF Bands No Yes