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Test Code PCR Bacti Bacterial PCR

Biohazard Infectious

Clinical System Name

Bacterial PCR

Synonyms

16s PCR

Tissue PCR

Body Fluid PCR

Bacterial Pathogen Detection by PCR - Tissue and Body Fluid

Bartonella sp PCR

Legionella sp PCR

M. tuberculosis PCR

Mycobacteria PCR

Description

Please forward all requests to Microbiology.

 

This test is helpful in diagnosis of culture negative infections.Tissue collected by surgical excision, normally sterile fluid, BAL, or abscess drainage material (when collected under sterile conditions) can be submitted for detection of bacteria by molecular methods. For in-house patients, a bacterial culture of the same sample must be submitted and confirmed as No Growth before the PCR testing will proceed. If a particular pathogen is suspected this information should be included in the Ordering comments. With bacterial growth, PCR testing will be cancelled. Consult with the Microbiology Director if PCR testing is still necessary.

 

For Cerebrospinal Fluids (CSF) PCR, see CSF Bacterial PCR for more requirements. PCR on CSF samples requires Infectious Disease approval.

 

External/Non-Children's ordering physicians must consult the Microbiology Director prior to sending any sample to Children's Microbiology Laboratory for PCR testing. 

Sample Requirements

Specimen: Tissue, Normally Sterile Fluid, BAL, or Abscess material

Container: Sterile Container

Preferred Vol: 1.0mL or grams

Minimum Vol: 0.5mL or grams

 

Swabs are not accetable and will be rejected. 

 

Processing Instructions

CPA: Send all samples to Microbiology for processing. If sample is shared and only one tube, syringe or other container is received, affix shared sample label and send entire sample plus requisition to Microbiology.

 

Off-site collection:  Off-site Children's Lab locations should send to SCH Main Campus for testing. If the sample has additional testing ordered, see the respective procedure for the appropriate storage and transport conditions.

 

For other off-site locations, sample should be stored frozen at -20C prior to and during transport to the SCH Microbiology Laboratory. 

Stability

On-Site Collection

Temp Time
Room temp 1 hour
Refrigerated N
Frozen N

* Off-site locations should store samples frozen at -20C prior to and during transport to the SCH Microbiology Laboratory. When shipping samples,

Availability

Procedure Performed Turn Around Time (TAT)
PCR 24/7

5 - 7 days

 

Performing Laboratory

Seattle Children's Laboratory

 

Department

Microbiology Laboratory

CPT Codes

Analyte Dependent

 

This test is not limited to the CPT codes listed.  Others may be added as more testing is necessary and depending on the pathogens isolated (such as MIC's and ID's)

Reporting Information

Preliminary Report:  48 hours

Final Report: 5 - 7 days

 

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:

http://www.doh.wa.gov/PublicHealthandHealthcareProviders/NotifiableConditions.aspx

 

See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

http://www.doh.wa.gov/Portals/1/Documents/1200/phsd-LHJ.pdf