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Test Code PCR Stl Diarrhea Rapid Stool Infectious Diarrhea PCR Pnl

Important Note

This orderable is only available to providers through appropriate PowerPlans.

Biohazard Infectious

Clinical System Name

Rapid Stool Infectious Diarrhea PCR Pnl

Synonyms

Stool testing

Stool PCR

Rapid Stool PCR

Stool Infectious Testing

Diarrhea

O&P

Ova and Parasite

Stool Viral Testing

Stool Culture

Rotavirus

Giardia

Norovirus

Adenovirus

Stool EIA

 

Description

The FilmArray Gastointestinal Panel (FA GI) is a multiplexed nucleic acid test designed for simultaneous qualitative detection and identification of multiple pathogens associated with gastrointestinal infection.

 

The infectious agents detected by this panel include:


Campylobacter (jejuni, coli, and upsaliensis)
Plesiomonas
Salmonella
Yersinia enterocolitica

E coli (enterotoxigenic E coli) ETEC
Shiga-like toxin-producing E coli (STEC) stx1/stx2

E coli O157

Shigella sp

Vibrio parahaemolyticus, vulnificus, and cholera
Cryptosporidium sp

Cyclospora sp

Entamoeba histolytica

Giardia

Adenovirus

Astrovirus

Norovirus

Rotavirus

Sapovirus

 

This test is NOT appropriate for test of cure. Following treatment, the patient can be retested by submitting stool for culture (C STOOL), or appropriate EIA testing.

 

This test should not be ordered more than ONCE per episode of diarrhea (at least 1 week between collections).

 

Sample Requirements

Specimen: Liquid or unformed stool collected in a clean leak-proof container is the optimal sample type. Rectal swabs are not optimal. In rare situations, exceptions may be made by contacting the Microbiology Lab 7-2585. Swab samples should be inserted at a distance sufficient to have visible stool on the swab (usually requires insertion of swab several centimeters into the rectum). Use the white capped E Swab System for collection.

 

Container: Clean leak-proof container

 

Preferred volume: 1 gram or ml

 

Minimum volume: 0.5 gram or ml

Processing Instructions

CPA: Send stool to Microbiology ASAP. If sample is shared and only one tube, syringe, or other container is received, affix shared sample label and send entire sample plus requisition to Microbiology. Please call Microbiology with sample related questions.

 

Off-Site Locations: This testing is only performed at SCH Microbiology. For satellite locations, the sample should be refrigerated prior to and during transport to the SCH Micro lab. Sample should be frozen at -20C for storage of >24 hours.

Stability

Specimen Temp Time
Fresh Stool Room Temp 2 hours
  Refrigerated ≤24 hours
  Frozen (-20C) 4 days

 

Availability

Procedure Performed Turn Around Time (TAT)
FilmArray PCR 24/7 2 hours

 

Performing Laboratory

Seattle Children's Laboratory

Department

Microbiology

Reference Range

Negative

 

Note: Samples that are PCR positive for the following bacterial pathogens will be plated for culture at no additional charge (for epidemiological purposes and/or susceptibility testing).
Plesiomonas

Salmonella

Yersinia

E coli O157

Shigella

Vibrio cholera, V parahaemolyticus, and V vulnificus

Methodology

Multiplex PCR

CPT Codes

87507

Notifiable Conditions

The following are notifiable conditions under Wa State Law:

Campylobacter, Cryptosporidium, Shiga toxin producing E coli (including but not limited to ECO157), Cyclospora, Giardia, Salmonella, Shigella, Vibrio, and Yersinia.

 

See Link below for Notifiable Conditions for WA state labs:

http://www.doh.wa.gov/PublicHealthandHealthcareProviders/NotifiableConditions.aspx

 

See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

http://www.doh.wa.gov/Portals/1/Documents/1200/phsd-LHJ.pdf