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Test Code PCR Stl GI Diar Rapid Stool GI IBD Bloody Diarrhea PCR

Important Note

This orderable is only available to providers through appropriate PowerPlans.

Biohazard Infectious

Clinical System Name

Rapid Stl GI IBD Bloody Diarrhea PCR Pnl

Synonyms

Stool testing

Stool PCR

Rapid Stool PCR

Stool Infectious Testing

Diarrhea

O&P

Ova and Parasite

C diff

Clostridium difficile

Giardia

Stool EIA

Stool Culture

 

 

 

Description

This rapid qualitative PCR test is appropriate for diagnosis of the infectious agents that are likely to be associated with Irritable Bowel Disease (IBD):

 

Agents included in this testing:

Campylobacter (jejuni, coli, and upsaliensis)

Clostridium difficile (toxin A/B)

Salmonella

Yersinia enterocolitica

Shiga-like toxin-producing E. coli (STEC) stx1/stx2

E. coli O157

Shigella

Vibrio parahaemolyticus, vulnificus, and cholerae

Entamoeba histolytica

Giardia lamblia

 

This test is NOT appropriate for test of cure. Following treatment, the patient can be re-tested by submitting stool for culture (C STOOL), or appropriate EIA Stl Protozoa orderable.

 

This test should not be ordered more than ONCE per episode of diarrhea (at least 1 week between collections).

 

 

Sample Requirements

Specimen: Liquid or unformed stool collected in a clean leak-proof container is the optimal sample type. Rectal swabs are not optimal. In rare situations, exceptions may be made by contacting the Microbiology Lab 7-2585. Swab samples should be inserted at a distance sufficient to have visible stool on the swab (usually requires insertion of swab several centimeters into the rectum). Use the white capped E Swab System for collection.

Container: Clean leak-proof container

 

Preferred volume: 1 gram or ml

 

Minimum volume: 0.5 gram or ml

 

Processing Instructions

CPA: Send stool to Microbiology ASAP. If sample is shared and only one tube, syringe, or other container is received, affix shared sample label and send entire sample plus requisition to Microbiology. Please call Microbiology with sample related questions.

 

Off-Site Locations: This testing is only performed at SCH Microbiology. For satellite locations, the sample should be refrigerated prior to and during transport to the SCH Micro lab. Sample should be frozen at -20C for storage of >24 hours.

Stability

Specimen Temp Time
Fresh Stool Room Temp 2 hour
  Refrigerated ≤ 24 hours
  Frozen 4 days

 

Availability

Procedure Performed

Turn Around Tim (TAT)

Stool PCR 24/7 2 hours

 

Performing Laboratory

Seattle Children's Laboratory

Department

Microbiology

Methodology

Multiplex PCR

CPT Code

87507

Reference Ranges

Negative

 

Note: Stool samples that are positive for the following bacterial pathogens will be cultured at no additional charge (for epidemiology and possible susceptibility testing):

Salmonella

Yersinia

E coli O157

Shigella

Vibrio parahaemolyticus, vulnificus, and cholerae

Notifiable Conditions

The following are notifiable conditions under Wa State Law:

Campylobacter, Shiga toxin producing E coli (including but not limited to EC O157), Giardia, Salmonella, Shigella, Vibrio, and Yersinia.

 

See Link below for Notifiable Conditions for WA state labs:

http://www.doh.wa.gov/PublicHealthandHealthcareProviders/NotifiableConditions.aspx

 

See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

http://www.doh.wa.gov/Portals/1/Documents/1200/phsd-LHJ.pdf