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Test Code PEP BF Electrophoresis, Protein, Body Fluid

Reporting Name

Electrophoresis, Protein, BF

Profile Information

Test ID Reporting Name Available Separately Always Performed
FLD4 Fluid Type No Yes
TP1 Protein, Total Yes, (TPBF) Yes
ELPBF Protein Electrophoresis, BF No Yes

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Body Fluid


Specimen Required


Container/Tube: Sterile container

Specimen Volume: 10 mL

Collection Instructions: Centrifuge to remove any cellular material.

Additional Information: Indicate specimen source; acceptable body fluid types are peritoneal (abdominal, ascites, paracentesis, peritoneal), pleural (chest, thoracentesis, pleural), drain (drainage, Jackson-Pratt (JP) drain), pericardial, spinal (CSF or ventricular), synovial (joint, knee, elbow), and vitreous fluids


Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time
Body Fluid Refrigerated (preferred) 7 days
  Frozen  14 days
  Ambient  72 hours

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Saturday; 12 p.m.

Test Classification

See Individual Test IDs

CPT Code Information

84166

LOINC Code Information

Result ID Test Result Name Result LOINC Value
2802 Albumin 12174-9
TP1 Protein, Total 2881-1
FLD4 Fluid Type 14725-6
2803 Alpha 1-Globulin 17812-9
2804 Alpha 2-Globulin 17814-5
2805 Beta-Globulin 17816-0
2806 Gamma-Globulin 17818-6

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Body fluid types other than those listed in Specimen Required

Method Name

Agarose Gel Electrophoresis

Useful For

Monitoring patient's body fluid proteins

 

Aiding in the diagnosis of monoclonal gammopathies, when used in conjunction with immunofixation of the patient's serum

 

Detecting oligoclonal banding in spinal fluid (the preferred test for detecting oligoclonal bands in spinal fluid is OLIG / Oligoclonal Banding, Serum and Spinal Fluid)

Method Description

Body fluids are separated in an electric field according to their size, shape, and electric charge using the Helena SPIFE 3000. The separation is performed on agarose gels (Helena SPIFE SPE Vis GEL). The proteins are visualized by staining with acid blue and the intensity of staining is quantitated by densitometry using the Helena Quick Scan 2000.(Helena SPIFE Instruction Manuel and Helena SPIFE SPE Vis Gel package insert 2001)