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Test Code PFGE Bacterial Typing by Pulsed-Field Gel Electrophoresis (PFGE)

Biohazard Infectious

Reporting Name

Bacterial Typing

Useful For

Investigating infection outbreaks by a single bacterial species


Advisory Information


This test identifies bacterial strains, except for Staphylococcus aureusStaphylococcus spp., not S. aureus; Listeria spp., Bacillus spp., Enterococcus spp., Streptococcus spp., Lactobacillus spp., Legionella spp., Enterobacteriaceae, non-Enterobacteriaceae, and Clostridium perfringens.

 

For Staphylococcus aureus isolates, order BTWGS / Bacterial Typing by Whole Genome Sequencing.



Shipping Instructions


See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.



Necessary Information


Specimen source and organism identification are required for processing.



Specimen Required


Supplies: Infectious Container, Large (T146)

Container/Tube: Agar slant

Specimen Volume: Isolates on separate agar slants in pure culture

Collection Instructions:

1. Organism must be in pure culture, actively growing. Do not submit mixed cultures.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.

3. Place all pulsed-field gel electrophoresis (PFGE) specimens (all patients and/or sites to be compared) together in a large bag, and send in the same shipping container. This is necessary for comparison of isolates by this method.

Additional Information:

1. Each slant must be submitted under a separate order.

2. Original isolates should be saved at the client site if additional PFGE testing may be needed (eg, to compare to future outbreak isolates that may occur).


Specimen Type

Varies

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Day(s) and Time(s) Performed

Monday through Friday; Continuously

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Pulsed-Field Gel Electrophoresis of DNA Fragments

Method Description

Typing of bacterial isolates is accomplished by comparing the DNA restriction fragment length polymorphism (RFLP) patterns of large DNA fragments separated by pulsed-field gel electrophoresis (PFGE) of 2 or more bacterial isolates. Identical RFLP patterns indicate similarity of the isolates. The test is performed by isolating the DNA from the organism, cutting the DNA with a restriction enzyme which recognize 10 to 20 sites on the genome, and separating the fragments using PFGE. The size pattern of DNA fragments in the electrophoresis gel of different isolates is then compared.(Tenover FC, Arbeit RD, Goering RV, et al: Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995;33:2233-2239)

Reference Values

Reported as isolates from these sources are "indistinguishable" or "different" by pulsed-field gel electrophoresis. Results will be faxed to the client.

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87152

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PFGE Bacterial Typing In Process

 

Result ID Test Result Name Result LOINC Value
PFGE Bacterial Typing In Process

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

NA