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Test Code PLT AGG Platelet Aggregation

Important Note

This is a specialty test which MUST be scheduled prior to collecting the sample. Testing is performed on specific scheduled days between the hours of 08:00  and 11:00 AM.

For patients arriving after 11:00 please contact the core lab coag team prior to drawing the patient, to determine if testing can be performed.

 

Do not collect samples for aggregation testing if the patient has taken aspirin, non-steroidal anti-inflammatory agents, or other platelet inhibiting medications within 10 days of testing as abnormal aggregation responses may occur.


Do NOT use tube system to transport samples, must be hand carried to the laboratory. Do not agitate the sample. Testing must be performed within 3 hours of collection. Sample cannot be used for any other testing.

Clinical System Name

Platelet Aggregation

Description

The Chrono - Log Model 700 is used to aid in the detection of platelet dysfunction using impedance to determine the ability of the platelets to aggregate, and luminescence to determine the qualitative functionality of the platelet granules.

Sample Requirements

Specimen: Whole blood

Container(s): Lt. Blue Top/Citrate, Lavender top /EDTA

Preferred Vol: (4) 2.7 mL Citrate tubes and (1) 4.0 mL EDTA tube

Minimum Vol:  (3) 2.7 mL Citrate tubes and (1) 4.0 mL EDTA tube

 

 

Note:This test MUST be scheduled with the core lab coagulation department prior to collection.  Samples will not be accepted if test is not scheduled in advance.

 

Do NOT use tube system to transport samples, must be hand carried to the laboratory. Do not agitate the sample. Testing must be performed within 3 hours of collection. Sample cannot be used for any other testing.

 

Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  A 21 gauge or larger needle is recommended, however if the patient is less than 4 years old, a smaller gauge needle can be used.  No syringe and collect a clearing tube first. Gently invert sample 4-5 times only. 

 

Collect a clearing tube, and then 4- 2.7ml blue top (3.2% sodium citrate) tubes, followed by a purple top (EDTA) tube. Place the citrate tubes in a rack in the order drawn.

 

 

 

Processing Instructions

Reject due to:

  • Hemolyzed, clotted or inadequately filled samples.
  • Specimen tubes sent to the lab via the pneumatic tube transport system.
  • Specimen tubes that have been refrigerated, centrifuged, or greater than 3 hours old.
  • Samples sent without prior scheduling with the performing department.

 

Spin: N

Aliquot: N

Temp: RT

Storage location: Core lab Coagulation department.

 

Note: Deliver blood to Core Heme bench (next to TEG rack).  Coag Technologist will process. 

Do not centrifuge specimen. Test should be set up within 3 hours of collection.

 

Off-site collection: Cannot be collected outside Children's. Contact Laboratory for additional information.

Stability

Specimen Type Temperature Time

Whole blood

Lt Blue/Citrate

Room temp 3 h
  Refrigerated N
  Frozen N

 

Availability

STAT Performed TAT
N As scheduled with Core Coag Dept. 1 d

 

 

Performing Laboratory

Seattle Children's Laboratory    

 

Department

Department:  Coagulation

Phone Number: 206 987-2578

 

 

Reference Range

Agonist Aggregation (ohm) ATP Release (nmol)
Collagen, 2 ug/mL 14 - 60 0.40 - 3.00
Collagen, 5 ug/mL 14 - 60 0.40 - 3.00
Arach. Acid, 0.5 mM 10 - 60 0.40 - 3.00
ADP, 5uM 6 - 60 N/A
ADP, 10uM 6 - 60 N/A
Ristocetin 0.25 mg/mL 0 - 2 N/A
Ristocetin 1.0 mg/mL 6 - 60 N/A

 

Methodology

Method:  Whole blood aggregometry by Chrono - Log model 700

 

 

Limitations: Method is affected by  improper handling. Do not use tube system to transport samples. Do not agitate samples, testing must be performed with in 3 hours.

 

CPT Codes

85576 x7

82397 x3

Critical Values

Not defined for this test.