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Test Code PLT FUNC S Platelet Function Screen

Important Note

Hour of availability are M - F  (07:30 - 17:00) No holidays or weekends. Requests outside hours of availability must be directed to the Core Coag department BEFORE drawing. All approved requests after 1500 must be called to x72561 to alert the lab that a platelet function screen will be arriving.

 

Do NOT use tube system to transport samples, must be hand carried to the laboratory. Do not agitate the sample. Testing must be performed with in 4 hours of collection. Sample cannot be used for any other testing.

Clinical System Name

Platelet Function Screen

Synonyms

PFS
PLT FUNC S
PLT Function Screen

Description

The PFA-100 can be used to detect platelet dysfunction. Platelet dysfunction may be acquired, inherited, or the result of platelet inhibiting agents. The most common causes of platelet dysfunction are related to uremia, von Willebrand disease, and exposure to medications such as acetylsalicyllic acid (aspirin) and clopidogrel (plavix).

Sample Requirements

Specimen: Whole blood

Container(s): Lt. Blue Top/Citrate

Preferred Vol: (4) 2.7 mL - please read instructions in note before drawing.

Minimum Vol:  (3) 2.7 mL  - please read instructions in note before drawing.

 

 

Note: Do NOT use tube system to transport samples, must be hand carried to the laboratory. Do not agitate the sample. Testing must be performed with in 4 hours of collection. Sample cannot be used for any other testing.

 

Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  23 gauge or larger needle. Draw blood directly into vacutainer tubes, no syringe draws. Invert sample 4-5 times only.

Use of a Vascular Access Device for the  collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

 

 

 

Processing Instructions

Reject due to:

  • Hemolyzed, clotted or inadequately filled samples are rejected.
  • Specimen tubes sent to the lab via the pneumatic tube transport system.
  • Specimen tubes that have been refrigerated, centrifuged, or greater than 4 hours old.

 

Spin: N

Aliquot: N

Temp: RT

Storage location: Core lab Coagulation department.

 

Note: Deliver blood to Core Heme bench (next to TEG rack).  Coag Technologist will process. 

Do not centrifuge specimen. Test should be set up  within 4 hours of collection.

 

Off-site collection: Cannot be collected outside Children's. Contact Laboratory for additional information.

Stability

Specimen Type Temperature Time

Whole blood

Lt Blue/Citrate

Room temp 4 h
  Refrigerated N
  Frozen N

 

Availability

STAT Performed TAT
N M-F  (07:30-17:00)  No holidays or Weekends 1 d

 

 *Requests outside these hours must be directed to the Core Coag department  BEFORE drawing. All approved requests after 1500 must be called to x72102 to alert the lab that a platelet function screen will be arriving.

Performing Laboratory

Seattle Children's Laboratory    

 

Department

Department:  Coagulation

Phone Number: 206 987-2578

 

 

Reference Range

  Seconds

Collagen/ADP Occlusion Time

≤ 114

Collagen/EPI Occlusion Time  

≤ 180

 

Methodology

Method:  Test cartridge system by Dade® PFA-100 Instrument.

 

Analytical Volume: 1.8 mL Whole blood

 

Limitations: Method is affected by  improper handling. Do not use tube system to transport samples. Do not aggitate samples, testing must be performed with in 4 hours.

 

CPT Codes

85576

Critical Values

Not defined for this test.