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Test Code PNH SCREEN Paroxysmal Nocturnal Hemoglobinuria Workup

Clinical System Name

PNH Work-up Request

Synonyms

CD 59
CD59
 

Description

This test is used in the workup for paroxysmal nocturnal hemoglobinuria (PNH), a rare hematopoietic stem cell disorder characterized by a somatic mutation in the PIGA gene, leading to a partial or absolute deficiency of proteins linked to the cell membrane via glycophosphatidylinositol (GPI) anchors.

Sample Requirements

Specimen:  Whole Blood

Container(s):  Lavender/EDTA

Preferred Vol:  2 mL

Minimum Vol:  1 mL

 

 

  Collected in-house at Seattle Children's Collected off-site (including SCH Bellevue)
M-Th collect any time collect any time
Friday collect any time ok if received at SCH main lab same day, if not do not collect
Saturday ok if collected before 12 noon do not collect
day before a holiday ok if collected before 12 noon must be received at SCH main lab before 12 noon
Sunday ok if collected after 12 noon (to be tested next morning)

ok if collected after 12 noon (to be tested next morning)

Sunday before a holiday Monday do not collect do not collect
Holiday ok if collected after 12 noon (to be tested next morning) ok if collected after 12 noon (to be tested next morning)

 

Processing Instructions

Reject due to:  n/a - send to lab

Spin: NO

Aliquot:  NO

Temp:  RT

Storage location:  Days:  Transport specimen and labels to 10th floor Cell Markers (station #280).    Eves/Nights:  Store specimen and labels in the Cell Markers RT box in CPA.

 

Off-site collection:  Keep whole blood at room temperature.

Stability

Temperature Time
Room temp 24 hours
Refrigerated

N

Frozen N

 

Availability

STAT Performed TAT
N performed M-F, Sat before noon 2-3 days

 

Note:   limited specimen stability, see sample requirements

Performing Laboratory

Seattle Children's Laboratory  

Department

Department:   Cell Markers

Phone Number: 206-987-2560

 

Reference Range

Reported with results.

Methodology

Method:  Flow Cytometry

Analytical Volume:  dependent on white count

Limitations: 

CPT Codes

contact Laboratory Client Services at (206)-987-2617