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Test Code Plt Recpt Platelet Receptor Workup by Flow Cytometry

Important Note

Off-site Collection: If specimen will not arrive at Children's before noon for same day testing, blood should be drawn as late in the day as possible, maintained at room temperature and sent overnight to allow test set-up the following morning. 

For further information, contact Hematopathology (Cell Markers) at 206-987-2560.

Children's Outpatient Blood Draw areas and Community Services - order "PLT RECPT" in Cerner and submit copy of requisition with specimen.

Clinical System Name

Platelet Receptor Workup by Flow Cytometry

Synonyms

Bernard-Soulier Platelet Characterization by Flow Cytometry

Glanzmann Thrombasthenia Platelet Characterization by Flow Cytometry

Description

Bernard-Soulier Syndrome and Glanzmann Thrombasthenia are rare autosomal recessive platelet disorders demonstrating aberrant expression of Gp1b and GpIIb/IIIa, respectively.  They can be characterized by evaluating platelet receptors by flow cytometry using monoclonal antibodies to CD41, CD42a and CD61.

Sample Requirements

Specimen:  Whole Blood

Container(s):  Lavender/EDTA; no microtainers accepted

Preferred Vol:  2 mL

Minimum Vol:  0.5 mL

 

Note:

Specimens stable for only 30 hours at room temperature.

 

 

  Collected in-house at Seattle Children's Collected off-site (including SCH Bellevue, South)
M-Th collect any time collect any time
Friday must be received before 12 noon Friday must be received at SCH main lab before 12 noon Friday
Saturday do not collect do not collect
day before a holiday ok if collected before 12 noon must be received at SCH main lab before 12 noon day before holiday
Sunday ok if collected after 12 noon (to be tested next morning)

ok if collected after 12 noon (to be tested next morning)

Sunday before a holiday Monday do not collect do not collect
Holiday ok if collected after 12 noon (to be tested next morning) ok if collected after 12 noon (to be tested next morning)

 

 

 

 

 

 

peripop     

Processing Instructions

Reject due to:  n/a - send to lab

Spin:  N - DO NOT SPIN

Aliquot:  N

Temp:  RT

Storage location:  Days: M - F until 1630, transport specimen and labels to 10th floor Cell Markers (station #280); call before tubing specimen to make sure there is someone available to do testing.

After hours: Store sample in Cell Markers RT box in CPA. 

 

Off-site collection:  Keep specimen at room temperature and transport to Children's Lab.  Please contact Laboratory Client Services at (206) 987-2617 with method of transport and shipping number.

 

 

Stability

Temperature Time
Room temp 30 hrs in EDTA
Refrigerated N
Frozen N

 

Availability

STAT Performed TAT
N

Monday - Friday 0800-1630

2-3 days

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Cell Markers

Phone Number: 206-987-2560

 

 

Reference Range

Interpretive report will be provided.
 

Methodology

Method:  Flow Cytometry

Analytical Volume:  100 microL

Limitations:  None specified

CPT Codes

 

  CPT CODE(S)
CM AB IF MALIGNANCY 1st Ab 88184
CM AB IF MALIGNANCY Add Ab 88185 x 2
CM PROFEE, Malignancy, 2-8 Markers 88187

 

For additional information, please contact Laboratory Client Services at (206)-987-2617