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Test Code Protein Total Protein

Important Note

Test uses serum only and is affected by all anticoagulants.

Clinical System Name

Total Protein


Total serum protein concentration can be used for evaluation of nutritional status. Causes of high total serum protein concentration include dehydration, Waldenström’s macroglobulinemia, multiple myeloma, hyperglobulinemia, granulomatous diseases, some tropical diseases, collagen diseases, lupus erythematosus, and other instances of chronic infection or inflammation. Low total serum protein concentration may be caused by excessive intravenous fluid administration, cirrhosis or other liver diseases, heart failure, nephrotic syndrome, glomerulonephritis, neoplasia, protein-losing enteropathies, malabsorption, and severe malnutrition.    

Sample Requirements

Specimen:  Whole Blood

Container(s):  Gold SST or Red

Preferred Vol:  1 mL

Minimum Vol:  1 Gold Microtainer


Note:  Method is affected by lipemia, hemolysis, and anticoagulants (any type).


Processing Instructions

Reject due to: Incorrect specimen type

Spin: Y

Aliquot: Y

Temp: 2-4oC

Storage location:  Core Chemistry Refrigerator


Off-site collection:  Avoid hemolysis. Do not use Dark Green/Sodium Heparin or any anticoagulant to collect specimen. Spin blood, separate serum from cells, and refrigerate at 2-4oC.



Specimen Type Temperature Time
Serum RT

4 H

Serum 2-4oC

3 D

Serum -20o C or -70oC

6 M



STAT Performed TAT
Y 24/7 1 H


Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Chemistry

Phone Number: 206-987-2617



Reference Range


0 - 1 week 4.4 - 7.6
1 week - 4 months 4.2 - 7.4
4 months - 1 year 5.6 - 7.2
1 year - 75 years 5.9 - 7.7



Method:  Colorimetric by Vitros 4600

Minimum Volume: 0.05 mL serum

Analytical Volume:  0.01 mL + 0.03 mL dead space

CPT Codes