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Test Code Protein Total Protein

Important Note

Test uses serum only and is affected by all anticoagulants.

Clinical System Name

Total Protein

Description

Total serum protein concentration can be used for evaluation of nutritional status. Causes of high total serum protein concentration include dehydration, Waldenström’s macroglobulinemia, multiple myeloma, hyperglobulinemia, granulomatous diseases, some tropical diseases, collagen diseases, lupus erythematosus, and other instances of chronic infection or inflammation. Low total serum protein concentration may be caused by excessive intravenous fluid administration, cirrhosis or other liver diseases, heart failure, nephrotic syndrome, glomerulonephritis, neoplasia, protein-losing enteropathies, malabsorption, and severe malnutrition.    

Sample Requirements

Specimen:  Whole Blood

Container(s):  Gold SST or Red

Preferred Vol:  1 mL

Minimum Vol:  1 Gold Microtainer

 

Note:  Method is affected by lipemia, hemolysis, and anticoagulants (any type).

 

Processing Instructions

Reject due to: Incorrect specimen type

Spin: Y

Aliquot: Y

Temp: 2-4oC

Storage location:  Core Chemistry Refrigerator

 

Off-site collection:  Avoid hemolysis. Do not use Dark Green/Sodium Heparin or any anticoagulant to collect specimen. Spin blood, separate serum from cells, and refrigerate at 2-4oC.

Stability

 

Specimen Type Temperature Time
Serum RT

4 H

Serum 2-4oC

3 D

Serum -20o C or -70oC

6 M

 

Availability

STAT Performed TAT
Y 24/7 1 H

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Chemistry

Phone Number: 206-987-2617

 

 

Reference Range

 

  G/dL
0 - 1 week 4.4 - 7.6
1 week - 4 months 4.2 - 7.4
4 months - 1 year 5.6 - 7.2
1 year - 75 years 5.9 - 7.7

 

Methodology

Method:  Colorimetric by Vitros 4600

Minimum Volume: 0.05 mL serum

Analytical Volume:  0.01 mL + 0.03 mL dead space

CPT Codes

84155