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Test Code Protph Fr Protoporphyrins, Fractionation, Whole Blood

Reporting Name

Protoporphyrins, Fractionation, WB

Useful For

Evaluating patients with possible diagnoses of erythropoietic protoporphyria or X-linked dominant protoporphyria

 

Establishing a biochemical diagnosis of erythropoietic protoporphyria and X-linked dominant protoporphyria

Testing Algorithm

This test should not be ordered in conjunction with the PEE / Porphyrins Evaluation, Whole Blood.

 

The following algorithms are available in Special Instructions:

-Porphyria (Acute) Testing Algorithm

-Porphyria (Cutaneous) Testing Algorithm

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Whole blood


Necessary Information


Include a list of medications the patient is currently taking.



Specimen Required


All porphyrin tests on whole blood can be performed on 1 draw tube.

 

Patient Preparation: Patient should abstain from alcohol for 24 hours.

Container/Tube:

Preferred: Green top (sodium heparin)

Acceptable: Dark blue top (metal free heparin) or green top (lithium heparin)

Specimen Volume: Full tube

Collection Instructions: Immediately place specimen on wet ice.


Specimen Minimum Volume

3 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole blood Refrigerated 7 days

Reference Values

FREE PROTOPORPHYRIN

<20 mcg/dL packed cells

 

ZINC-COMPLEXED PROTOPORPHYRIN

<60 mcg/dL packed cells

Day(s) and Time(s) Performed

Monday through Friday; 8 a.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

82542

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PPFE Protoporphyrins, Fractionation, WB In Process

 

Result ID Test Result Name Result LOINC Value
2327 Zinc-Complexed Protoporphyrin 33007-6
2326 Free Protoporphyrin 2892-8
29511 Interpretation 59462-2

Method Description

Extraction followed by fractionation by high-performance liquid chromatography. Zinc protoporphyrin and free protoporphyrin are separately quantitated.(Smith RM, Doran D, Mazur M, Bush B: High-performance liquid chromatographic determination of protoporphyrin and zinc protoporphyrin in blood. J Chromatogr 1980 Mar 14;181[3-4]:319-327)

Reject Due To

Hemolysis

Mild reject; Gross reject

Lipemia

NA

Icterus

NA

Other

NA

Method Name

High-Performance Liquid Chromatography (HPLC) with Fluorescence Detection

Forms

New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (T576) is available in Special Instructions.