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Test Code Pyru Ki Qn Pyruvate Kinase, Erythrocytes

Reporting Name

Pyruvate Kinase, RBC

Useful For

Evaluation of nonspherocytic hemolytic anemia

 

Evaluation of neonatal anemia

 

Evaluation of unusually severe hemoglobin S trait

 

Evaluation of unusually severe glucose-6-phosphate dehydrogenase deficiency

 

Investigating families with pyruvate kinase deficiency to determine inheritance pattern and for genetic counseling


Specimen Required


Container/Tube:

Preferred: Yellow top (ACD solution B)

Acceptable: EDTA

Specimen Volume: 6 mL

Collection Instructions: Do not transfer blood to other containers.


Specimen Type

Whole Blood ACD-B

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood ACD-B Refrigerated 20 days

Day(s) and Time(s) Performed

Monday through Saturday

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Kinetic Spectrophotometry (KS)

Method Description

A red cell hemolysate is incubated with adenosine diphosphate and phosphoenolpyruvate. The amount of pyruvate formed is quantitated by adding lactic dehydrogenase and reduced nicotinamide adenine dinucleotide and measuring the rate of decrease in absorbance at 340 nm.(Beutler E: Red Cell Metabolism. In A Manual of Biochemical Methods. Third edition. New York, Grune and Stratton, 1984, pp 68-71)

Reference Values

≥12 months: 6.7-14.3 U/g Hb

Reference values have not been established for patients who are <12 months of age.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

84220

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PK Pyruvate Kinase, RBC In Process

 

Result ID Test Result Name Result LOINC Value
PK_ Pyruvate Kinase, RBC 32552-2

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

NA

Icterus

NA

Other

NA

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)