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Coenzyme Q10, Reduced and Total, Plasma

Reporting Name

Coenzyme Q10, Reduced and Total, P
Seattle Children's Hospital Note:

Clinical System Name: Miscellaneous Test

Useful For

Diagnosis of coenzyme Q10 (CoQ10) deficiency in mitochondrial disorders


Monitoring patients receiving statin therapy


Monitoring CoQ10 status during treatment of various degenerative conditions including Parkinson and Alzheimer disease

Advisory Information

This test provides both reduced and total coenzyme Q10. For assessment of total only, order TQ10 / Coenzyme Q10, Total, Plasma.


The level of oxidized Q10 was affected in specimens with even slight amounts of hemolysis; however, the total Q10 level remains constant. Hemolyzed specimens can be analyzed for total Q10 using TQ10 / Coenzyme Q10, Total, Plasma.

Specimen Required

Patient Preparation: Fasting (8 hours)

Collection Container/Tube: Green top (lithium or sodium heparin)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Immediately place specimen on wet ice. Maintain on wet ice and process within 3 hours of draw.

2. Spin down, separate plasma from cells, and immediately freeze specimen.

Specimen Type

Plasma Heparin

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Heparin Frozen (preferred) 72 hours
  Refrigerated  8 hours

Day(s) and Time(s) Performed

Monday through Friday; 8 a.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection

Method Description

Coenzyme Q10 (CoQ10) is extracted from plasma with cold 1-propanol containing coenzyme Q9 as an internal standard. An aliquot of the lipid extract is fractionated by high-performance liquid chromatography (HPLC). The separated coenzyme Q10 reduced and coenzyme Q10 oxidized are quantified by measurement of selective coulometric response emerging from the chromatographic column.(Tang PH, Miles MV, DeGrauw A, et al: HPLC analysis of reduced and oxidized coenzyme Q[10] in human plasma. Clin Chem 2001 February;47[2]:256-265)

Reference Values


<18 years: 320-1,376 mcg/L

≥18 years: 415-1,480 mcg/L



<18 years: 320-1,558 mcg/L

≥18 years: 433-1,532 mcg/L



<18 years: 93-100%

≥18 years: 92-98%


Miles MV, Horn PS, Tang PH, et al: Age-related changes in plasma coenzyme Q10 concentrations and redox state in apparently healthy children and adults. Clin Chim Acta 2004;34:139-144

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
Q10 Coenzyme Q10, Reduced and Total, P In Process


Result ID Test Result Name Result LOINC Value
87853 CoQ10 reduced 81157-0
30091 CoQ10 Total 27923-2
30092 CoQ10 % reduced 81156-2
30159 Interpretation 59462-2

Reject Due To


Mild reject; Gross reject


Mild reject; Gross reject


Mild OK; Gross OK