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Test Code QF TB Quantiferon-TB Gold Test

Clinical System Name

Quantiferon-TB Gold

Sample Requirements

Specimen: Whole blood

Container(s): 3 Quantiferon TB Tubes: Nil, TB Antigen, and Mitogen

Preferred Vol: Fill to black mark on each tube (1 mL per tube)

Minimum Vol: N/A


Note: Obtain special Quantiferon tubes and specific instructions from Laboratory (987-2102).  If butterfly needle is used, a "purge" tube should be collected to ensure tubing is filled before filling Quantiferon TB Gold tubes. After collection, shake tubes 10 times to coat the inner surface and deliver to the laboratory at RT.


Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temp: RT

Storage location:




00:00 – 8:00


Store in Biochemical Genetics RT box

08:00 – 16:00

M – F

Tube to OC.10

Sa – Su,


Invert tubes 10 times and store upright in rack in CPA incubator.

Note incubation time start time on label.

16:00 – 23:59


Invert tubes 10 times and store upright in rack in CPA incubator under.

Note incubation time start time on label.


Off-site collection:  Specimens must be collected in three Quantiferon TB Gold tubes and received within 16 h of collection.  Ship at RT.


Specimen Type Temperature Time
  Room temp 16 h
  Refrigerated N
  Frozen N



STAT Performed TAT
N T, F 1 w

Call the Chemistry laboratory (206-987-2565) for requests outside of stated availability. Because of the incubation time necessary for testing, results cannot be obtained prior to 36-48 hr post blood draw.

Performing Laboratory

Seattle Children's Laboratory    



Department:  Chemistry

Location: Chem West

Phone Number: (206) 987-2617




Reference Range

Expected value: Negative

Patients on steroids may have indeterminate results. For indeterminate-low mitogen results a redraw is recommended.


Method: Interferon-gamma release assay

Analytical Volume:


CPT Codes