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Test Code Retic Reticulocyte

Clinical System Name

Reticulocyte Count

Description

The circulating reticulocyte count is the most convenient method for evaluating effective marrow production of erythrocytes.

Sample Requirements

Specimen: Whole Blood

Container(s):  Lavender/EDTA

Preferred Vol:  1.0-3.0 mL

Minimum Vol:  0.5 mL microtainer; 1.0 ml macrotainer

 

Notes: A Lavender top/EDTA vacutainer must contain at least 1.0 mL.

Processing Instructions

Reject due to:  Insufficient Quantity, Wrong Sample Collection, Clotted Sample

Spin: N

Aliquot: N

Temp: RT

Storage location:  Deliver to Core Lab Hematology bench.

 

Off-site collection: Store whole blood at room temperature and transport to Children's Lab immediately.  Samples > 24 hours old may be rejected.

Stability

 

Specimen Type Temperature Time
Whole blood RT

24 hrs

Whole blood 2-8oC

 48 hrs

Whole blood -20oC or -70oC

N/A

 

Availability

STAT Performed TAT
Y 24/7 1 H

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Hematology

Phone Number: 206-987-2561

 

 

Reference Range

 

Reticulocyte

 

 

Ret-He*

   

Raw Retic ABS

 

0 - 1 Day

2.3 - 5.1%

 

0 - Adult

28 pg - 38 pg

  0 - Adult

 0.018 – 0.100 M/mm3 

1 Day - 1 Week

0.6 - 1.4%

 

 

 

 

1- 4 Weeks

0.8 - 1.4%

 

 

 

 

4 - 6 Weeks

0.8 - 2.2%

 

 

 

 

6 - 8 Weeks

0.8 - 2.7%

 

 

 

 

8 - 10 Weeks

1.0 - 2.4%

 

 

 

 

10 - 12 Weeks

0.9 - 1.5%

 

 

 

 

12 Weeks - Adult

1.0 - 2.0%

 

 

 

 

*Reticulocyte hemoglobin equivalent (Ret-He) is a parameter dependent on the hemoglobin content of the reticuloctyes. Ret-He is usually decreased in patients with iron deficiency anemia (IDA). IDA could be diagnosed with a sensitivity of 93% and specificity of 83% when the Ret-He is low.

 

 

Methodology

Method:  Sysmex XN-3000 & XN-1000: Flow cytometry with semiconductive laser

Analytical Volume:  0.025 mL  plasma (no guarantee)

 

CPT Codes

85044