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Test Code SCHISTO AB Schistosoma species Antibody, IgG, Serum

Useful For

Detection of antibodies to Schistosoma species

Method Name

Enzyme Immunoassay (EIA)

Reporting Name

Schistosoma Ab, IgG, S

Specimen Type

Serum


Specimen Required


Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 1 mL


Specimen Minimum Volume

0.50 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 30 days
  Frozen  30 days

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

NA

Other

NA

Reference Values

Negative

Method Description

The qualitative immunoenzymatic determination of IgG-class antibodies against Schistosoma mansoni is based on the Enzyme-Linked Immunosorbent Assay (ELISA) technique.

 

Microtiter strip wells are precoated with Schistosoma mansoni antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material, horseradish peroxidase (HRP)-labelled Protein A conjugate is added. This conjugate binds to antigen-antibody complexes. The immune complex formed by the bound conjugate is visualized by adding tetramethylbenzidine (TMB) substrate, which gives a blue reaction product.

 

The intensity of this product is proportional to the amount of Schistosoma-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.(Package insert: NovaLisa Schistosoma mansoni, NovaTec Immundiagnostica GmbH, Dietzenbach, Germany)

Day(s) and Time(s) Performed

Tuesday, Thursday; 9 a.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

86682

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BILHA Schistosoma Ab, IgG, S 33317-9

 

Result ID Test Result Name Result LOINC Value
BILHA Schistosoma Ab, IgG, S 33317-9