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Test Code Sickle Scrn Sickle Cell Screen

Clinical System Name

Sickle Cell Screen

Description

The Sickle Cell Screen is a qualitative screening test for the detection of hemoglobin S in blood samples. The test is used for the diagnosis of Sickle cell disease in conjunction with other criteria.  A hematocrit is run concurrently. Hemoglobin electrophoresis is automatically ordered to confirm positive sickle results.

 

False negatives may occur due to fetal hemoglobin; this test should not be performed on children under the age of 6 months. Suggest hemoglobin electrophoresis instead.

Sample Requirements

Specimen: Whole Blood

Container(s):  Lavender/EDTA

Preferred Vol:  1.0-3.0 mL

Minimum Vol:  1.0 mL

 

Notes: A minimum of two Lavender top/EDTA microtainers.

Processing Instructions

Reject due to:  Insufficient Quantity, Wrong Sample Collection, Clotted Sample

Spin: N

Aliquot: N

Temp: RT

Storage location:  Deliver to Core Lab Hematology bench.

 

Off-site collection: Store whole blood at 2-8oC and transport to Children's Lab immediately.  Samples viable for 2 weeks

when stored at 2-8oC.

Stability

 

Specimen Type Temperature Time
Whole Blood RT

No

Whole Blood 2-8oC

2 wks

Whole Blood -20oC or -70oC

N/A

 

Availability

STAT Performed TAT
Y 24/7 1 H

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Hematology

Phone Number: 206-987-2561

 

 

Reference Range

Negative

 

Methodology

Method:   Pacific Hemostasis® SickleScreen® Sickling Hemoglobin Screening Kit 

Analytical Volume:  0.025 mL  plasma (no guarantee)

 

CPT Codes

85660