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Test Code Sickle Scrn Sickle Cell Screen

Clinical System Name

Sickle Cell Screen


The Sickle Cell Screen is a qualitative screening test for the detection of hemoglobin S in blood samples. The test is used for the diagnosis of Sickle cell disease in conjunction with other criteria.  A hematocrit is run concurrently. Hemoglobin electrophoresis is automatically ordered to confirm positive sickle results.


False negatives may occur due to fetal hemoglobin; this test should not be performed on children under the age of 6 months. Suggest hemoglobin electrophoresis instead.

Sample Requirements

Specimen: Whole Blood

Container(s):  Lavender/EDTA

Preferred Vol:  1.0-3.0 mL

Minimum Vol:  1.0 mL


Notes: A minimum of two Lavender top/EDTA microtainers.

Processing Instructions

Reject due to:  Insufficient Quantity, Wrong Sample Collection, Clotted Sample

Spin: N

Aliquot: N

Temp: RT

Storage location:  Deliver to Core Lab Hematology bench.


Off-site collection: Store whole blood at 2-8oC and transport to Children's Lab immediately.  Samples viable for 2 weeks

when stored at 2-8oC.



Specimen Type Temperature Time
Whole Blood RT


Whole Blood 2-8oC

2 wks

Whole Blood -20oC or -70oC




STAT Performed TAT
Y 24/7 1 H


Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Hematology

Phone Number: 206-987-2561



Reference Range




Method:   Pacific Hemostasis® SickleScreen® Sickling Hemoglobin Screening Kit 

Analytical Volume:  0.025 mL  plasma (no guarantee)


CPT Codes