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Test Code St Calprotn Calprotectin, Stool

Clinical System Name

Calprotectin, Stool

Synonyms

Fecal Calprotectin

Sample Requirements

Specimen: Stool

Container(s):  Sterile Screw-Capped Container

Preferred Vol: 5 grams

Minimum Vol:  1 gram

 

Note: Indicate collection date and time. Diaper collection must be removed from diaper by family and sent in sterile container.

Processing Instructions

Reject due to: Stool mixed with media or preservatives; stool submitted in diapers or plastic bags (must be transferred to sterile containers by family).

Spin: N

Aliquot: N

Temp: 2-8 C

Storage location: Separate specimens must be aliquoted when multiple tests are ordered. Maintain sterility.

Deliver sample to CPA Refrigerator, FrigG

Off-site collection:  Ship refrigerated.

Stability

Specimen Type Temperature Time
Stool Room temp

5 d

  Refrigerated 5 d
  Frozen

1 yr

 

Availability

STAT Performed TAT
N M 2 - 7 d

 

Performing Laboratory

Seattle Children's Laboratory

Department

Department: Chemistry

Location: Chem East 

Phone Number: 206-987-2102

 

 

 

Reference Range

 

50 mg/kg or less Normal
51-120 mg/kg Borderline elevated, test should be re-evaluated in 4-6 weeks.
121 mg/kg or greater Positive; Abnormal*

 

*An abnormal result indicates the presence of PMNs in stool and is suggestive of inflammation. This result along with other clinical findings is can be used to diagnose IBD.

Methodology

Method: ELISA

Analytical Volume: None specified

Limitations:

1.    Bloody stool samples should not affect patient results per Inova Technical Services.
2.    False-negative results could occur in patients who have granulocytopenia due to bone marrow depression.
3.    Some patients who are taking NSAIDs will have elevations in their fecal calprotectin levels.
4.    Patients with IBD fluctuate between active (inflammatory) and inactive stages of the disease. These stages must be considered when using the QUANTA Lite Calprotectin assay.
5.    The use of proton pump inhibitors (PPIs), microscopic colitis and diverticular disease may also lead to elevated calprotectin level. Patients affected by untreated celiac disease may occasionally show elevated calprotectin value.
6.    Other intestinal impairments, including many gastrointestinal infections and colorectal cancer, can result in elevated levels of calprotectin. These specimens will test positive with the QUANTA Lite Calprotectin assay. Therefore, a diagnosis of active IBD cannot be established solely on the basis of a positive result with the QUANTA Lite Calprotectin assay.
7.    Fecal calprotectin is an indicator of neutrophilic presence in the stool and is not specific for IBD.
 

CPT Codes

83993