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Test Code T Cell Phen T-Cell Subsets, Naive, Memory and Activated

Important Note

Sample may only be collected Monday to Thursday.

 

Seattle Children's Order Code: T Cell Phenotyping

Reporting Name

T Cell Phenotyping, Advanced
Seattle Children's Hospital Note:

Clinical System Name:  T Cell Phenotyping

Useful For

Determining the presence of naive, memory, and activated T cells in various clinical contexts including autoimmune diseases, immunodeficiency states, T-cell recovery posthematopoietic stem cell transplant, DiGeorge syndrome, and as a measure for T-cell immune competence

 

Naive T-cells results can be used as a surrogate marker for thymic-derived T-cell reconstitution, when used in conjunction with assessment of T-cell receptor excision circles (TREC / T-Cell Receptor Excision Circles [TREC] Analysis for Immune Reconstitution)

 

Assessing a patient's relative risk for infections

 

Evaluation of patients with cellular or combined primary immunodeficiencies

 

Evaluation of T-cell reconstitution after hematopoietic stem cell transplant, chemotherapy, biological therapy, immunosuppression or immunomodulator therapy

 

Evaluation of patients with autoimmune diseases

 

Evaluation of HIV-positive patients for naive and memory subsets

 

Evaluation of T-cell immune competence (presence of memory and activated T cells) in patients with recurrent infections

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Whole Blood EDTA


Specimen Required


Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. It is recommended that specimens arrive within 24 hours of draw. Specimens arriving on the weekend may be canceled. Draw and package specimen as close to shipping time as possible.

 

Container/Tube: Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions: Send specimen in original tube. Do not aliquot.

Additional Information:

1. Ordering physician's name and phone number are required.

2. For serial monitoring, we recommend that specimen draws be performed at the same time of day.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood EDTA Ambient 72 hours

Reference Values

The appropriate age-related reference values will be provided on the report.

Day(s) and Time(s) Performed

Monday through Friday

Do not send specimen after Thursday. Specimen must be received by 10 a.m. on Friday.

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

86359-T cells, total count

86361-Absolute CD4 count

88184-Flow cytometry

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TCP T Cell Phenotyping, Advanced In Process

 

Result ID Test Result Name Result LOINC Value
29151 %CD4+CD45RA+ naive T cells 13336-3
29152 %CD4+CD62L+CD27+ naive T cells In Process
29153 %CD8+CD45RA+ naive T cells In Process
29154 %CD8+CD62L+CD27+naive T cells In Process
29155 %CD4+CD45RO+ memory T cells 41994-5
29156 %CD4+CD62L+CD27+CD45RO+ (Tcm) In Process
29157 %CD4+CD62L-CD27-CD45RO+ (Tem) In Process
29158 %CD8+CD45RO+ memory T cells In Process
29159 %CD8+CD62L+CD27+CD45RO+ (Tcm) In Process
29160 %CD8+CD62L-CD27-CD45RO+ (Tem) In Process
29161 %Activated CD4 T cells (4+CD25+) 13332-2
29162 %CD4+HLA DR+CD28+ T cells In Process
29163 %CD8+HLA DR+CD28+ T cells In Process
29164 CD4+CD45RA+ naive T cells 26759-1
29165 CD4+CD62L+CD27+ naive T cells In Process
29166 CD8+CD45RA+ naive T cells In Process
29167 CD8+CD62L+CD27+naive T cells In Process
29168 CD4+CD45RO+ memory T cells 34475-4
29169 CD4+CD62L+CD27+CD45RO+ (Tcm) In Process
29170 CD4+CD62L-CD27-CD45RO+ (Tem) In Process
29171 CD8+CD45RO+ memory T cells In Process
29172 CD8+CD62L+CD27+CD45RO+ (Tcm) In Process
29173 CD8+CD62L-CD27- CD45RO+ (Tem) In Process
29174 Activated CD4 T cells (4+CD25+) 26982-9
29175 CD4+HLA DR+CD28+ T cells In Process
29176 CD8+HLA DR+CD28+ T cells In Process
29178 Interpretation 69052-9

Method Description

This flow cytometric assay quantitates the following CD4 and CD8 T-cell subsets: naive (global and CD62L+), memory (global, central, and effector memory), and activated (CD4+25+ and MHC class II-positive) T cells. EDTA anticoagulated blood is incubated with antibodies to various T-cell markers (ie, CD3, CD4, CD8, CD45RO, CD45RA, HLADR, CD27, CD62L, CD25, and CD28). After RBC lysis, the sample is washed to remove any unbound antibodies prior to analysis on a BD FACS Cantoinstrument. The assay uses 4 antibody tubes for data acquisition and analysis is performed as 3 panels. Each T-cell subset is expressed as a percentage of total CD4+ or CD8 T cells. Only the CD3 T cells are expressed as a percentage of total lymphocytes. The absolute counts for the T-cell subsets are derived from flow cytometry analysis of whole blood using monoclonal antibodies to identify CD45, CD3, CD4, and CD8. CD14 is used to exclude monocytes, thereby improving accuracy and enhancing the purity of the lymphocyte population. The assay is a 5-color, lyse-no wash procedure and the absolute counts are calculated from internal bead standards. The T-cell subsets panel is linked to the TCD4 test (TCD4 / CD4 Count for Immune Monitoring, Blood) within the experiment and, therefore, the CD3, CD4, and CD8 T-cell reference ranges are provided within the TCD4 assay. The results for the other T-cell subsets are interpreted using a reference range derived from data of normal healthy adult and pediatric donors. Isotype controls are used in each assay to measure background fluorescence of the samples. A normal, healthy control is also included in each experiment to ensure the optimal performance of the assay.(Unpublished Mayo information)

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

NA

Other

NA

Method Name

Flow Cytometry