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Test Code TClon UM T Cell Clonality

Clinical System Name

T Cell Clonality

Synonyms

Gamma chain

T Cell Receptor

Description

Monoclonal populations of T lymphocytes can be identified by testing for T cell receptor gamma chain (TCRG) gene rearrangements using a multiplex polymerase chain reaction assay followed by fragment analysis by capillary electrophoresis. A polyclonal T cell population will produce a Gaussian distribution of PCR products on capillary electrophoresis, while a clonal process will produce one or two discrete peaks. Approximately 95% of T cell lymphomas produce clonal PCR products using our primer set. A neoplastic clone must represent at least 1% of the total T cells in a mixed population for a clonal peak to be detectable in a polyclonal distribution of background peaks.

 

Sample Requirements

Specimen: Whole Blood, Bone Marrow, or Tissue

Container(s): Lavender/EDTA (preferred) or Dk Green/Sodium Heparin

Preferred Vol: 6 mL Whole Blood; 1 - 2 mL Bone Marrow

Minimum Vol:

 

Note: While green top tubes are accepted for testing, there is documentation that heparin can interfere with some PCR assays.

Processing Instructions

Reject due to: N/A - send to lab

Spin: N

Aliquot: N

Temp: 2 - 8 C

Storage location: CPA1, Send Outs rack

 

Note:

Optimal results are achieved when samples are received within: 72 hours of collection for bone marrow and peripheral blood   24 hours of collection for CSF, body fluids, and fresh tissue  

Stability

Specimen Type Temperature Time
Whole Blood or Bone Marrow Room temp  
  Refrigerated 72 h
  Frozen

 

 

Specimen Type Temperature Time
CSF, Body Fluids, or Fresh Tissue in RPMI Room temp  
  Refrigerated 24 h
  Frozen  

 

Availability

STAT Performed TAT
N Tuesday  

 

Performing Laboratory

UW Molecular Hematopathology

825 Eastlake Avenue East, Rm G7-800
Seattle, WA 98195
 

Phone: (206) 288-7060

Department

Department: Send Outs

Phone Number: (206) 987-2563

Methodology

Method: Polymerase Chain Reaction (PCR)/Capillary Electrophoresis

Analytical Volume:

Limitations:

CPT Codes

81342

Send Out Instructions

Reference Test Name T Cell Clonality by PCR
Reference Test Number TCRG
Instructions Send out M-F with the UW courier. Keep a copy of the batch sheet for our records. Weekends and Holidays courier to UW NW220.