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Test Code TOBRA, TOBRA TR, TOBRA PK Tobramycin - Non-Cystic Fibrosis Patients

Important Note

No add-ons after 8 hours of collection if specimen is still on gel separator.

Clinical System Name

Tobramycin Level (Random, Peak, Trough)

Sample Requirements

Specimen:  Whole Blood

Container(s):  Gold SST, Red, Dark Green/Sodium Heparin, or Lavender/EDTA

Preferred Vol: 1 mL

Minimum Vol:  1 Lt. Green/Li Heparin Microtainer,1 Gold Microtainer, or 1 Lavender/EDTA Microtainer

 

Note:  Every 8 hour dosing: Infuse IV over 30 minutes. Draw peak level after 3rd or 4th dose 30 minutes after the end of the IV infusion. Trough levels should be drawn 30 minutes before the 4th dose. Document the date and time of infusion and collections. Also, document whether the sample is a peak, a trough, or a random level.

 

Processing Instructions

Reject due to:  Specimen type not indicated (e.g. random, trough, peak, etc.), Insufficient quantity

Spin: Y

Aliquot: Y

Temp: 2-4oC

Storage location:  Core Chemistry Refrigerator

 

Off-site collection:  Every 8 hour dosing: Infuse IV over 30 minutes. Draw peak level after 3rd or 4th dose 30 minutes after the end of the IV infusion. Trough levels should be drawn 30 minutes before the 4th dose. Document the date and time of infusion and collections. Also, document whether the sample is a peak, a trough, or a random level. Spin blood, freeze plasma or serum.

Stability

 

Specimen Type Temperature Time
Serum or Plasma RT

2 H

Serum or Plasma 2-4oC

7 D

Serum or Plasma -20o C or -70oC

14 D

 

Availability

STAT Performed TAT
Y 24/7 1 H

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Chemistry

Phone Number: 206-987-2617

 

 

Reference Range

 

  mcg/mL
Peak 4 - 12
Trough 0 - 2
Toxic >12

 

Methodology

Method:  Two Point Rate method by Vitros 4600

Minimum Volume: 0.1 mL serum or plasma

Analytical Volume: 0.06 mL serum or plasma + 0.03 mL dead space

Critical Values

> 12 mcg/mL

CPT Codes

80200