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Test Code U ALA Aminolevulinic Acid (ALA), Urine

Reporting Name

Aminolevulinic Acid, U
Seattle Children's Hospital Note:

Clinical System Name: Aminolevulinic Acid (ALA), Urine

Useful For

Assistance in the differential diagnosis of the various acute hepatic porphyrias

Testing Algorithm

The following algorithms are available in Special Instructions:

-Porphyria (Acute) Testing Algorithm

-Porphyria (Cutaneous) Testing Algorithm

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type


Necessary Information

Patient's age is required.

Specimen Required

Patient Preparation: Patient should abstain from alcohol for 24 hours prior to and during testing.

Supplies: Urine Tubes, 10 mL (T068)

Specimen Volume: 2 mL

Collection Instructions: Collect a random urine specimen.

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Urine Refrigerated (preferred) 28 days
  Frozen  45 days

Reference Values

<1 year: ≤10 nmol/mL

1-17 years: ≤20 nmol/mL

≥18 years: ≤15 nmol/mL

Day(s) and Time(s) Performed

Tuesday, Thursday; 8 a.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
ALAUR Aminolevulinic Acid, U 34284-0


Result ID Test Result Name Result LOINC Value
61547 Aminolevulinic Acid, U 34284-0
34347 Interpretation (ALA), U 59462-2
34348 Reviewed By In Process

Method Description

Aminolevulinic acid (ALA) is determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) stable isotope dilution analysis. The urine is mixed with an internal standard (5-aminolevulinic acid, 13C5, 15N, ALA-IS) and filtered using a 0.2 mcM nylon filter vial. The ratios of the extracted peak areas of ALA to ALA-IS determined by LC-MS/MS are used to calculate the concentration of ALA present in the sample.(Lacey, JM, Magera MJ, and Tortorelli S: Delta Aminolevulinic Acid Quantitation in Urine by LC-MS/MS. J Am Soc Mass Spectrom 2011;22, S1:pp 69)

Reject Due To









Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)


New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (T576) is available in Special Instructions.