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Test Code U Hyperoxal Hyperoxaluria Panel, Urine

Additional Codes

Glycolate

 

Reporting Name

Hyperoxaluria Panel, U

Useful For

Distinguishing between primary and secondary hyperoxaluria

 

Distinguishing between primary hyperoxaluria types 1, 2, and 3


Specimen Required


Patient Preparation: Fasting-overnight (12-14 hours).

Supplies: Urine Tubes, 10 mL (T068)

Container/Tube: Plastic, 10-mL urine tube (T068)

Specimen Volume: 10 mL

Collection Instructions:

1. Have patient void the first-morning specimen, then collect specimen within 2 hours of first-morning void while patient continues to fast. Fluids are allowed.

2. No preservative.

3. Immediately freeze specimen.


Specimen Type

Urine

Specimen Minimum Volume

1.1 mL

Specimen Stability Information

Specimen Type Temperature Time
Urine Frozen (preferred) 90 days
  Refrigerated  14 days

Day(s) and Time(s) Performed

Thursdays; 8 a.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Gas Chromatography-Mass Spectrometry (GC-MS)

Method Description

Urine samples corresponding to 0.25 mg of creatinine (not to exceed 1 mL of urine) are oximated by reaction with methoxyamine hydrochloride to stabilize one of the target analytes, 4-hydroxy-2-oxoglutaric (HOG). The urine is then acidified and extracted with 4:1 ethyl acetate:isopropanol. After evaporation, the dry residue is silylated with 80:20 BSTFA/1%TMCS:pyridine and analyzed by capillary gas chromatography/mass spectrometry (GC/MS). In the context of hyperoxaluria, per se a non-specific finding, the detection of elevated excretions of urinary glycolate and glycerate allows the differential diagnosis between hyperoxaluria type 1 and type 2. Increased concentrations of oxalate and glycolate indicate type1 hyperoxaluria. Increased concentrations of oxalate and glycerate indicate type 2 hyperoxaluria. Increased concentration of HOG indicates type 3 hyperoxaluria, or carrier status for this disease. Increased concentrations of oxalate with normal concentrations of glycolate, glycerate, and HOG indicate secondary hyperoxaluria.(Unpublished Mayo method)

Reference Values

REPORTING/INTERPRETING RESULTS

Reference Intervals (Normal Ranges):

 

Glycolate

≤17 years: ≤75 mg/g creatinine

≥18 years: ≤50 mg/g creatinine

 

Glycerate

≤31 days: ≤75 mg/g creatinine

32 days - 4 years: ≤125 mg/g creatinine

5 - 10 years: ≤55 mg/g creatinine

≥11 years: ≤25 mg/g creatinine

 

Oxalate

≤6 months: ≤400 mg/g creatinine

7 months - 1 year: ≤300 mg/g creatinine

2 - 6 years: ≤150 mg/g creatinine

7 - 10 years: ≤100 mg/g creatinine

≥11 years: ≤75 mg/g creatinine

 

4-hydroxy-2-oxoglutarate (HOG)

10 mg/g creatinine

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

82542

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HYOX Hyperoxaluria Panel, U 53710-0

 

Result ID Test Result Name Result LOINC Value
50592 Glycolate 13751-3
50593 Glycerate 13749-7
50594 Oxalate 13483-3
38049 4-hydroxy-2-oxoglutarate In Process
29982 Interpretation 59462-2
29984 Reviewed By No LOINC Needed

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Any preservative

Testing Algorithm

See Hyperoxaluria Diagnostic Algorithm in Special Instructions.

Forms

New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (T576) is available in Special Instructions.