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Test Code Vit B2 Riboflavin (Vitamin B2), Plasma

Useful For

Evaluation of persons who present the signs of ariboflavinosis

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Riboflavin (Vitamin B2), P
Seattle Children's Hospital Note:

Clinical System Name: Vitamin B2 Level

Specimen Type

Plasma Heparin

Shipping Instructions

Send specimen in amber vial to protect from light.

Specimen Required

Patient Preparation: Fasting-overnight (12-14 hours) (infants-draw prior to next feeding).

Supplies: Amber Frosted Tube, 5 mL (T192)

Collection Container/Tube: Green top (heparin)

Submission Container/Tube: Amber vial (T192)

Specimen Volume: 0.5 mL

Collection Instructions:

1. Place heparin collection tube on ice, keep covered.

2. Centrifuge and aliquot to amber vial

3. Freeze immediately

Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Heparin Frozen (preferred) 14 days
  Refrigerated  7 days

Reject Due To


Mild OK; Gross OK


Mild OK; Gross reject


Mild OK; Gross OK


EDTA or serum

Reference Values

Normal: 1-19 mcg/L

Method Description

The stable isotope riboflavin 13C15N2 is added to plasma as an internal standard. Meta-phosphoric acid solution is then added to precipitate the proteins. Following sedimentation of the proteins, an aliquot of the clarified supernatant fluid is subjected to separation of riboflavin and internal standard from other plasma components by reverse-phase HPLC with quantitation by tandem mass spectrometry (LC-MS/MS).(Unpublished Mayo methods)

Day(s) and Time(s) Performed

Monday, Wednesday, Friday; Evening

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
VITB2 Riboflavin (Vitamin B2), P 2924-9


Result ID Test Result Name Result LOINC Value
61637 Riboflavin (Vitamin B2), P 2924-9