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Clinical System Name
Miscellaneous Test Communication Order
Synonyms
OncoPlex Cancer Gene Panel
OPX
Description
UW-OncoPlex is a multiplexed gene sequencing panel that detects mutations in tumor tissue in 262 cancer-related genes (listed in the methods below). The panel includes genes related to cancer treatment, prognosis, and diagnosis. The test uses next-generation "deep" sequencing to detect most classes of mutations, including single nucleotide variants, small insertions and deletions (indels), gene amplifications, and selected gene-fusions. In addition, microsatellite instability status is reported for colorectal cancers.
This test is designed to detect somatic mutations in cancer, and is not designed to detect germline (heritable) mutations.
Sample Requirements
Specimen Type: Peripheral Blood, Bone Marrow, Tumor Tissue, Purified DNA
Tissue Samples: Send EITHER (a) tissue
block, OR (b) curls, OR (c) slides:
(a) Instructions for tissue block specimen:
Provide complete tissue block containing tumor tissue.
If there is more than one tissue block, please provide the block
that has the greatest amount of tumor tissue. Tissue block will be
returned at completion of testing.
(b) Instructions for specimen curls:
1 slide at 4-micron thickness stained with hematoxylin-and-eosin
AND
15 curls at 10-micron thickness
Note: Curls should contain at least 20% tumor tissue.
(c) Instructions for slide specimens:
1 slide at 4-micron thickness stained with hematoxylin-and-eosin
AND
15 unstained, non-baked slides at 10-micron thickness (a minimum of
10 unstained slides is acceptable).
Unstained slides can be on charged or uncharged slides.
Note: Sections should contain at least 20% tumor tissue.
Purified DNA: 2 microgram. Reference
hematoxylin-and-eosin stained slide and pathology report
required.
Blood: 6 mL blood in LAVENDER TOP (EDTA)
tube.
Bone Marrow: 1 to 2 mL Bone Marrow in
LAVENDER TOP (EDTA) tube.
Unacceptable: Decalcified samples; tissue samples treated with fixatives other than formalin.
Note: If collecting bone marrow, the sample must be drawn in a Lavender/EDTA tube.
Processing Instructions
Reject due to: Frozen or clotted specimens
Spin: N
Aliquot: N
Temp: Refrigerated
Storage location: Affix large Cerner label(s) to tube(s) and place in refrigerated Send Outs rack. Store extra labels and a copy of requisition in the clear plastic box attached to CPA refrigerator. Alert Send Outs team.
Off-site collection: Send whole blood refrigerated
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood | Room temp | 3 d |
| Whole Blood | Refrigerated | 7 d |
| Whole Blood | Frozen |
N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | 4 - 6 w |
Performing Laboratory
University of Washington Medical Center
Department of Laboratory Medicine
Genetics Lab
1959 NE Pacific St, NW220
Seattle, WA 98195
Phone Number: (206) 520-4600
Department
Department: Send Outs
Phone: (206) 987-2563
Methodology
Method: UW-OncoPlex is a multiplexed gene sequencing panel that detects mutations in tumor tissue in 262 cancer-related genes (listed in the methods below). The panel includes genes related to cancer treatment, prognosis, and diagnosis. The test uses next-generation "deep" sequencing to detect most classes of mutations, including single nucleotide variants, small insertions and deletions (indels), gene amplifications, and selected gene-fusions. In addition, microsatellite instability status is reported for colorectal cancers.
Send Out Instructions
| Reference Test Name: | UW OncoPlex Cancer Gene Panel |
| Reference Test Number: | OPX |
| Instructions: | Send out Monday - Friday with the UW courier. |
UWMC Genetics Lab page:
http://depts.washington.edu/labweb/Divisions/MolDiag/MolDiagGen/index.htm
UWMC Genetics Lab requisition:
http://depts.washington.edu/labweb/referencelab/clinical/TestForms/genetics.pdf