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HelpOncoPlex
Synonyms
OncoPlex Cancer Gene Panel
OPX
Description
UW-OncoPlex is a multiplexed mutation assay for tumor tissue that assesses mutations in 262 genes related to cancer treatment, prognosis, or diagnosis. The genes are sequenced on an Illumina instrument to detect single nucleotide variants, insertions, deletions, gene amplifications, and selected translocations. Microsatellite instability (MSI) status is reported for colorectal cancer cases. This test is designed to detect somatic mutations in cancer, and is not designed to detect germline (heritable) mutations.
Sample Requirements
Specimen: Whole blood, Bone Marrow, or Purified DNA
Container(s): Lavender/EDTA
Preferred Vol: 6 mL
Minimum Vol: 3mL
Specimen: Tissue samples: Send EITHER (a) tissue block, OR (b) curls, OR (c) slides:
Container(s):
Preferred Vol:
Minimum Vol:
Note: If collecting bone marrow, the sample must be drawn in a Lavender/EDTA tube. Various tissue samples are also acceptable. For more information, see UW Medicine LabMed link at the bottom of this page.
Unacceptable: Decalcified samples; tissue samples treated with fixatives other than formalin.
Processing Instructions
Reject due to: Frozen or clotted specimens
Spin: N
Aliquot: N
Temp: Refrigerated
Storage location: Affix large Cerner label(s) to tube(s) and place in refrigerated Send Outs rack. Store extra labels and a copy of requisition in the clear plastic box attached to CPA refrigerator. Alert Send Outs team.
Off-site collection: Send whole blood refrigerated
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood | Room temp | 3 d |
| Whole Blood | Refrigerated | 7 d |
| Whole Blood | Frozen |
N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| No | 4 - 6 w |
Performing Laboratory
University of Washington
Dept of Laboratory Medicine
UW Genetics Lab
1959 NE Pacific St, NW220
Seattle, WA 98195
Phone Number: (206) 520-4600
Department
Department: Send Outs
Phone: (206) 987-2563
Methodology
Method: UW-OncoPlex is a multiplexed gene sequencing panel that detects mutations in tumor tissue in 262 cancer-related genes (listed in the methods below). The panel includes genes related to cancer treatment, prognosis, and diagnosis. The test uses next-generation "deep" sequencing to detect most classes of mutations, including single nucleotide variants, small insertions and deletions (indels), gene amplifications, and selected gene-fusions. In addition, microsatellite instability status is reported for colorectal cancers.
Analytical Volume: 3 mL for Whole Blood or Bone Marrow, 3 mcg of Purified DNA
Limitations:
Send Out Instructions
| Reference Test Name | UW OncoPlex Cancer Gene Panel |
| Reference Test Number | OPX |
| Instructions | Send out M - F with the UW courier. |
UWMC Genetics Lab page:
http://depts.washington.edu/labweb/Divisions/MolDiag/MolDiagGen/index.htm
UWMC Genetics Lab requisition:
http://depts.washington.edu/labweb/referencelab/clinical/TestForms/genetics.pdf
Clinical System Name
Miscellaneous Test Communication Order