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OncoPlex

Clinical System Name

Miscellaneous Test Communication Order

Synonyms

OncoPlex Cancer Gene Panel

OPX

Description

UW-OncoPlex is a multiplexed gene sequencing panel that detects mutations in tumor tissue in 262 cancer-related genes (listed in the methods below). The panel includes genes related to cancer treatment, prognosis, and diagnosis. The test uses next-generation "deep" sequencing to detect most classes of mutations, including single nucleotide variants, small insertions and deletions (indels), gene amplifications, and selected gene-fusions. In addition, microsatellite instability status is reported for colorectal cancers.

 

This test is designed to detect somatic mutations in cancer, and is not designed to detect germline (heritable) mutations.

Sample Requirements

Specimen Type: Peripheral Blood, Bone Marrow, Tumor Tissue, Purified DNA

 

Tissue Samples:  Send EITHER (a) tissue block, OR (b) curls, OR (c) slides:
(a) Instructions for tissue block specimen:
Provide complete tissue block containing tumor tissue.
If there is more than one tissue block, please provide the block that has the greatest amount of tumor tissue. Tissue block will be returned at completion of testing.
(b) Instructions for specimen curls:
1 slide at 4-micron thickness stained with hematoxylin-and-eosin AND
15 curls at 10-micron thickness
Note: Curls should contain at least 20% tumor tissue.
(c) Instructions for slide specimens:
1 slide at 4-micron thickness stained with hematoxylin-and-eosin AND
15 unstained, non-baked slides at 10-micron thickness (a minimum of 10 unstained slides is acceptable).
Unstained slides can be on charged or uncharged slides.
Note: Sections should contain at least 20% tumor tissue.

 

Purified DNA: 2 microgram. Reference hematoxylin-and-eosin stained slide and pathology report required.

Blood:  6 mL blood in LAVENDER TOP (EDTA) tube.

Bone Marrow:  1 to 2 mL Bone Marrow in LAVENDER TOP (EDTA) tube.

Unacceptable:  Decalcified samples; tissue samples treated with fixatives other than formalin.

Note: If collecting bone marrow, the sample must be drawn in a Lavender/EDTA tube.

Processing Instructions

Reject due to: Frozen or clotted specimens

Spin: N

Aliquot: N

Temp: Refrigerated

Storage location: Affix large Cerner label(s) to tube(s) and place in refrigerated Send Outs rack. Store extra labels and a copy of requisition in the clear plastic box attached to CPA refrigerator. Alert Send Outs team.

 

Off-site collection: Send whole blood refrigerated

Stability

Specimen Type Temperature Time
Whole Blood Room temp 3 d
Whole Blood Refrigerated 7 d
Whole Blood Frozen

N

 

Availability

STAT Performed TAT
N   4 - 6 w

 

Performing Laboratory

University of Washington Medical Center

Department of Laboratory Medicine

Genetics Lab
1959 NE Pacific St, NW220
Seattle, WA 98195

 

Phone Number: (206) 520-4600

Department

Department: Send Outs

Phone: (206) 987-2563

Methodology

Method: UW-OncoPlex is a multiplexed gene sequencing panel that detects mutations in tumor tissue in 262 cancer-related genes (listed in the methods below). The panel includes genes related to cancer treatment, prognosis, and diagnosis. The test uses next-generation "deep" sequencing to detect most classes of mutations, including single nucleotide variants, small insertions and deletions (indels), gene amplifications, and selected gene-fusions. In addition, microsatellite instability status is reported for colorectal cancers.

Special Instructions

UWMC

Requisition

Send Out Instructions

Reference Test Name: UW OncoPlex Cancer Gene Panel
Reference Test Number: OPX
Instructions: Send out Monday - Friday with the UW courier.

 

UWMC Genetics Lab page:

http://depts.washington.edu/labweb/Divisions/MolDiag/MolDiagGen/index.htm

 

UWMC Genetics Lab requisition:

http://depts.washington.edu/labweb/referencelab/clinical/TestForms/genetics.pdf