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Test Code A1 ATRY PH Alpha-1-Antitrypsin Phenotype

Reporting Name

Alpha-1-Antitrypsin Phenotype

Useful For

Identification of homozygous and heterozygous phenotypes of the alpha-1-antitrypsin deficiency

Specimen Required


Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 1.25 mL

Seattle Children's Hospital Note:

Collect 2.5 mL whole blood in a Red top.

Specimen Type


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Day(s) and Time(s) Performed

Monday through Friday; 9 a.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

A1AP2: Isoelectric Focusing

AATP: Nephelometry

Method Description

Phenotyping is done by isoelectric focusing in agarose gels.(Package insert: Hydragel 18 A1AT Isofocusing. Sebia. 2013 July)


Nephelometry.(Instruction manual: Siemens Nephelometer II. Siemens, Inc., Newark, DE)

Reference Values


100-190 mg/dL



The interpretive report will identify the alleles present. For rare alleles, the report will indicate whether or not they have been associated with reduced quantitative levels of alpha-1-antitrypsin.

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


82104-Alpha-1-antitrypsin phenotype

LOINC Code Information

Test ID Test Order Name Order LOINC Value
A1APP Alpha-1-Antitrypsin Phenotype 32769-2


Result ID Test Result Name Result LOINC Value
AATP Alpha-1-Antitrypsin, S 6771-0
8166 Alpha-1-Antitrypsin Phenotype 32769-2

Testing Algorithm

See Alpha-1-Antitrypsin-A Comprehensive Testing Algorithm in Special Instructions.

Reject Due To


Mild OK; Gross OK


Mild OK; Gross reject


Mild OK; Gross OK



Profile Information

Test ID Reporting Name Available Separately Always Performed
A1AP2 Alpha-1-Antitrypsin Phenotype No Yes
AATP Alpha-1-Antitrypsin, S Yes, (Order AAT) Yes


If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Test Request Form (T728) with the specimen (