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Test Code ALDOSTER U Aldosterone, 24 Hour, Urine

Reporting Name

Aldosterone, U

Useful For

Investigation of primary aldosteronism (eg, adrenal adenoma/carcinoma and adrenal cortical hyperplasia) and secondary aldosteronism (renovascular disease, salt depletion, potassium loading, cardiac failure with ascites, pregnancy, Bartter syndrome)


Advisory Information


Advice on stimulation or suppression tests is available from Mayo Clinic's Division of Endocrinology and may be obtained by calling Mayo Medical Laboratories.



Necessary Information


24-Hour volume is required.



Specimen Required


Patient Preparation: Spironolactone (Aldactone) should be discontinued for 4 to 6 weeks before testing.

Supplies: Urine tubes, 10-mL (T068)

Submission Container/Tube: Plastic, urine tube (T068)

Specimen Volume: 10 mL

Collection Instructions:

1. Collect urine for 24 hours.

2. Add 25 mL of 50% acetic acid as preservative at start of collection. Use 15 mL of 50% acetic acid for children <5 years old. This preservative is intended to achieve a pH of between approximately 2 and 4.

Additional Information: See Urine Preservatives for multiple collections and Renin-Aldosterone Studies for more detailed instructions in Special Instructions.


Specimen Type

Urine

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Urine Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  14 days

Day(s) and Time(s) Performed

Tuesday, Thursday; 1 p.m.

Seattle Children's Hospital Note:

TAT: 4 - 10 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Method Description

Samples are spiked with deuterated internal standard and are hydrolyzed overnight with acid. Samples are then neutralized and extracted by solid phase extraction (SPE). The extracts are dried, reconstituted, and analyzed by LC-MS/MS.(Taylor RL, Singh RJ: Validation of liquid chromatography-tandem mass spectrometry method for analysis of urinary conjugated metanephrine and normetanephrine for screening of pheochromocytoma. Clin Chem 2002 Mar;48[3]:533-539)

Reference Values

0-30 days: 0.7-11.0 mcg/24 hours*

31 days-11 months: 0.7-22.0 mcg/24 hours*

≥1 year: 2.0-20.0 mcg/24 hours

 

*Loeuille GA, Racadot A, Vasseur P, Vandewalle B: Blood and urinary aldosterone levels in normal neonates, infants and children. Pediatrie 1981;36:335-344

 

For SI unit Reference Values, see https://www.mayomedicallaboratories.com/order-tests/si-unit-conversion.html.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

82088

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ALDU Aldosterone, U 1765-7

 

Result ID Test Result Name Result LOINC Value
8556 Aldosterone, U 1765-7
TM47 Collection Duration 13362-9
VL45 Urine Volume 3167-4

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

NA

Urine Preservative Collection Options

Ambient

Yes

Refrigerated

Yes

Frozen

Yes

6N HCl

No

50% Acetic Acid

Preferred

Na2CO3

No

Toluene

Yes

6N HNO3

No

Boric Acid

Yes

Thymol

No