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HelpTest Code AMOEBA IGG Entamoeba histolytica Antibody, Serum
Reporting Name
E. histolytica Ab, SUseful For
As an adjunct in the diagnosis of extraintestinal amebiasis, especially liver abscess
Serology may be particularly useful in supporting the diagnosis of amebic liver abscess in patients without a definite history of intestinal amebiasis and who have not spent substantial periods of time in endemic areas
Advisory Information
Direct detection of Entamoeba histolytica in stool specimens is recommended to diagnose intestinal amebiasis. See OAP / Parasitic Examination.
Shipping Instructions
Specimen Required
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 0.5 mL
Collect 1.0 mL whole blood in a Red top.
Short stability, do not draw Wednesday evenings or Thursdays. Any questions call send outs at 987-2563.
Specimen Type
SerumSpecimen Minimum Volume
0.15 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen (preferred) | 30 days |
| Refrigerated | 7 days |
Day(s) and Time(s) Performed
Tuesday, Thursday; 9 a.m.
TAT: 3 - 9 days
Performing Laboratory
Mayo Medical Laboratories in Rochester
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Method Description
Purified antigens are coated to a microwell plate. Antibodies in the patient samples bind to the antigens and are determined during the second step by using enzyme-labelled Protein A (the conjugate). The enzyme converts the colourless substrate (urea peroxide/TMB) to a blue end product. The enzyme reaction is stopped by adding sulphuric acid and the colour of the mixture switches from blue to yellow at the same time. The final measurement is carried out at 450 nm on a photometer using a reference wavelength ≥620 nm.(Package insert: RIDASCREEN Entamoeba histolytica IgG, R-Biopharm AG, Darmstadt, Germany, 06/10/2016)
Reference Values
Negative
Test Classification
This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86753
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| SAM | E. histolytica Ab, S | 22285-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 9049 | E. histolytica Ab, S | 22285-1 |
Reject Due To
|
Hemolysis |
Mild OK; Gross reject |
|
Lipemia |
Mild OK; Gross reject |
|
Icterus |
NA |
|
Other |
NA |
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
Gastroenterology and Hepatology Test Request Form (T728) (http://www.mayomedicallaboratories.com/it-mmfiles/gastroenterology-and-hepatology-test-request.pdf)
Microbiology Test Request Form (T244) (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf)