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Test Code AMOEBA IGG Entamoeba histolytica Antibody, Serum

Reporting Name

E. histolytica Ab, S

Useful For

As an adjunct in the diagnosis of extraintestinal amebiasis, especially liver abscess


Ordering Guidance


Direct detection of Entamoeba histolytica in fecal specimens is recommended to diagnose intestinal amebiasis. See OAP / Ova and Parasite, Concentrate and Permanent Smear, Microscopy, Feces or OAPNS / Ova and Parasite, Microscopy, Varies.



Shipping Instructions


 



Specimen Required


Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Specimen Type

Serum

Specimen Minimum Volume

0.15 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen (preferred) 30 days
  Refrigerated  7 days

Day(s) Performed

Tuesday, Thursday

Seattle Children's Hospital Note:

TAT: 3 - 9 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Method Description

Purified antigens are coated to a microwell plate. Antibodies in the patient samples bind to the antigens and are determined during the second step by using enzyme-labelled protein A (the conjugate). The enzyme converts the colorless substrate (urea peroxide/TMB) to a blue end product. The enzyme reaction is stopped by adding sulfuric acid and the colour of the mixture switches from blue to yellow at the same time. The final measurement is carried out at 450 nm on a photometer using a reference wavelength greater than or equal to 620 nm.(Package insert: RIDASCREEN Entamoeba histolytica IgG. R-Biopharm AG; 07/10/2019)

Reference Values

Negative

Reference values apply to all ages.

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86753

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SAM E. histolytica Ab, S 22285-1

 

Result ID Test Result Name Result LOINC Value
9049 E. histolytica Ab, S 22285-1

Report Available

Same day/1 to 5 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.