Test Code AMOEBA IGG Entamoeba histolytica Antibody, Serum
Reporting Name
E. histolytica Ab, SUseful For
As an adjunct in the diagnosis of extraintestinal amebiasis, especially liver abscess
Ordering Guidance
Direct detection of Entamoeba histolytica in fecal specimens is recommended to diagnose intestinal amebiasis. See OAP / Ova and Parasite, Concentrate and Permanent Smear, Microscopy, Feces or OAPNS / Ova and Parasite, Microscopy, Varies.
Shipping Instructions
Specimen Required
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 0.5 mL
Specimen Type
SerumSpecimen Minimum Volume
0.15 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Frozen (preferred) | 30 days | |
Refrigerated | 7 days |
Day(s) Performed
Tuesday, Thursday
TAT: 3 - 9 days
Performing Laboratory

Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Method Description
Purified antigens are coated to a microwell plate. Antibodies in the patient samples bind to the antigens and are determined during the second step by using enzyme-labelled protein A (the conjugate). The enzyme converts the colorless substrate (urea peroxide/TMB) to a blue end product. The enzyme reaction is stopped by adding sulfuric acid and the colour of the mixture switches from blue to yellow at the same time. The final measurement is carried out at 450 nm on a photometer using a reference wavelength greater than or equal to 620 nm.(Package insert: RIDASCREEN Entamoeba histolytica IgG. R-Biopharm AG; 07/10/2019)
Reference Values
Negative
Reference values apply to all ages.
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86753
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
SAM | E. histolytica Ab, S | 22285-1 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
9049 | E. histolytica Ab, S | 22285-1 |
Report Available
Same day/1 to 5 daysReject Due To
Gross hemolysis | Reject |
Gross lipemia | Reject |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.