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Test Code AMY BF Body Fluid Amylase

Clinical System Name

Body Fluid Amylase

Sample Requirements

Specimen:  Body Fluid

Container(s): Sterile Screw-Capped Container, Sterile Screw-Capped Tube or Syringe, Gold SST, Red

Preferred Vol: 0.5 mL

Minimum Vol: 0.25 mL body fluid


Note: Note type of body fluid on requisition. Abdominal, ascites, body, chest, pericardial, and peritoneal fluid is acceptable.  Aliquot 0.5 mL body fluid into a plastic bullet tube and centrifuge.

Processing Instructions

Reject due to: Age of Specimen, Incorrect specimen container

Spin: Y

Aliquot: Y

Temp: 2-8oC

Storage location: Core 5 Chemistry Refrigerator

Notes: Chemistry scientist should refer to procedure for blood amylase if fluid is proteinaceous. Otherwise, refer to amylase urine procedure.


Off-site collection: Note type of body fluid on requisition. Centrifuge 0.5 mL body fluid; transfer supernatant to plastic aliquot tube. Refrigerate 2-8oC.



Specimen Type Temperature Time
Body Fluid RT

7 D

Body Fluid 2-8oC

 30 D

Body Fluid -20oC or -70oC

Not Recommended



STAT Performed TAT
Y 24/7 1 H


Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Chemistry

Phone Number: 206-987-2617

Reference Range

Reference ranges are not available for BF.


Method:  Spectrophotometric, Rate methods by Vitros 4600

Analytical Volume:  0.02 mL supernatant + 0.03 mL dead space

CPT Codes