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Test Code ARGATROBAN ARGATROBAN

Clinical System Name

Argatroban
 

Synonyms

DTI

Direct Thrombin Inhibitor

 

Description

Argatroban is used for prophylaxis or treatment of thrombosis in patients with heparin induced thrombocytopenia (HIT). The quantitative determination of plasma Argatroban levels is useful for monitoring treatment efficacy.

Sample Requirements

Specimen: Whole Blood

Container(s): Lt Blue/Citrate

Preferred Vol: 1.8 mL

Minimum Vol: N

 

Note:  Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the  collection of coag testing is not recommended.

 

If a venipuncture is not possible and drawing from a line is necessary:

a. Draw from a line not used to infuse argatroban if possible. 

b. If drawing from a line used to infuse argatroban, waste the following volume:

   A 6-mL waste volume for PIVs, PICCs, temporary venous lines (femoral, jugular) and Baby Broviacs.

  A 12-mL waste volume for all other permanent lines (Hickman, Portacath, HD, tunneled lines).

Processing Instructions

Reject due to: Clotted, Hemolyzed, Insufficient quantity, and  Improper collection.

Spin: N

Aliquot: N

Temp: RT

Storage location: Core 6 Freezer  ( -70 C).

 

Note: Deliver blood to Core Coag bench.  Coag Technologist will process. Specimen should be centrifuged within 1 hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label.

 

 

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended.

 

If a venipuncture is not possible and drawing from a line is necessary:

a. Draw from a line not used to infuse argatroban if possible. 

b. If drawing from a line used to infuse argatroban, waste the following volume:

   A 6-mL waste volume for PIVs, PICCs, temporary venous lines (femoral, jugular) and Baby Broviacs.

  A 12-mL waste volume for all other permanent lines (Hickman, Port-a-cath, HD, tunneled lines).

 

Specimen should be centrifuged within 1 hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze one aliquot of 1.0 mL plasma in plastic tube at -70C.

Ship frozen on dry ice.

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen   -20 C or -70 C 3 m

 

Availability

STAT Performed TAT
Y

Daily

1 h

 

Performing Laboratory

Seattle Children's Laboratory    

 

Department

Department:  Coagulation

Phone Number: 206 987-2578

 

 

 

Reference Range

Therapeutic Range : 0.5-2.0  µg/mL.

Methodology

Method: Electromagnetic mechanical clot detection system by STA® - Compact Analyzer

Analytical Volume: 1 mL

This is enough for a PT, PTT, TT, and Fibrinogen on the same sample.

 

Limitations: Method is affected by improper collection, processing and storage.

High hematocrit greater than or equal to 56% requires a citrate adjusted tube.

CPT Codes

80299

Special Instructions

Links to:

Consent Forms

Algorithms

Critical Values

Not defined for this test.