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HelpTest Code BCR/ABL p190 BCR/ABL p190, Quantitative PCR
Clinical System Name
BCR/ABL p190, Quant
Synonyms
B-ALL
BALL
B-Cell Acute Lymphobastic Leukemia
P190
Philadelphia chromosome
QP190
Quantitative BCR/ABL p190
t(9;22)
translocation (9;22)
Sample Requirements
Specimen: Whole Blood OR Bone Marrow
Container(s): Lavender/EDTA
Preferred Vol: 10.0 mL Whole Blood OR 2.0 mL Bone Marrow
Minimum Vol: 6.0 mL Whole Blood OR 1.0 mL Bone Marrow
Note: Record specimen type and date/time of collection on label.
For more detailed info for bone marrow testing, see separate Bone Marrow Aspirate listing.
Processing Instructions
Reject due to:
Spin: N
Aliquot: N
Temp: 2 - 4 C
Notes: Affix large label to vacutainer.
Storage Location: Store sample in CPA refrigerator Send Outs rack. On weekends, send sample refrigerated to UW NW220 ASAP so that sample can be run first thing Monday morning.
Off-site Collection: Contact UW Hematopathology at (206) 288-7060 for instructions.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Room temp | <1 d | |
| Refrigerated | 72 h | |
| Frozen | Unacceptable |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | Th | 2 w |
Performing Laboratory
University of Washington Medical Center
Hematopathology Laboratory
G7-800, Seattle Cancer Care Alliance
825 Eastlake Avenue E.
Seattle, WA 98109
Phone Number: (206) 606-7060
Department
Department: Send Outs
Phone Number: (206) 987-2563
Methodology
Method: LDT, RT-PCR
Analytical Volume: 6.0 mL Blood OR 1.0 - 2.0 mL Bone Marrow
Limitations:
Reference Range
See individual components.
Send Out Instructions
| Reference Test Name: | BCR/ABL p190, Quant by RT PCR |
| Reference Test Number: | QP190 |
| Instructions: | Send out Monday through Friday with the UW courier. |
Description
The Philadelphia chromosome (Ph) is the product of the reciprocal translocation between the ABL1 gene on chromosome 9, and the BCR gene on chromosome 22. Depending on variations in splicing, the BCR exon 1 can join to the ABL1 exon 2, resulting in the p190 (e1:a2) BCR/ABL1 breakpoint. p190 is found almost exclusively in Ph+ALL patients, but can, on occasion, be found in CML patients as an alternative splice variant. Our p190 assay is a laboratory-developed test using real- time quantitative PCR. This assay is able to detect one Ph+ cell in 10,000 normal cells. Samples with low mutational burden may appear as wild-type.