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Test Code BCR/ABL p190 BCR/ABL p190, Quantitative PCR

Clinical System Name

BCR/ABL p190, Quant

Synonyms

B-ALL

BALL

B-Cell Acute Lymphobastic Leukemia

P190

Philadelphia chromosome

QP190

Quantitative BCR/ABL p190

t(9;22)

translocation (9;22)

Description

The Philadelphia chromosome (Ph) is the product of the reciprocal translocation between the ABL1 gene on chromosome 9, and the BCR gene on chromosome 22. Depending on variations in splicing, the BCR exon 1 can join to the ABL1 exon 2, resulting in the p190 (e1:a2) BCR/ABL1 breakpoint. p190 is found almost exclusively in Ph+ALL patients, but can, on occasion, be found in CML patients as an alternative splice variant. Our p190 assay is a laboratory-developed test using real- time quantitative PCR. This assay is able to detect one Ph+ cell in 10,000 normal cells. Samples with low mutational burden may appear as wild-type.

Sample Requirements

Specimen: Whole Blood OR Bone Marrow

Container(s): Lavender/EDTA

Preferred Vol: 10.0 mL Whole Blood OR 2.0 mL Bone Marrow

Minimum Vol: 6.0 mL Whole Blood OR 1.0 mL Bone Marrow

 

Note: Record specimen type and date/time of collection on label.

For more detailed info for bone marrow testing, see separate Bone Marrow Aspirate listing.

Stability

Specimen Type Temperature Time
  Room temp <1 d
  Refrigerated 72 h
  Frozen Unacceptable

 

Availability

STAT Performed TAT
N Th 2 w

Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temp: 2 - 4 C

Notes: Affix large label to vacutainer.

Storage Location: Store sample in CPA refrigerator Send Outs rack. On weekends, send sample refrigerated to UW NW220 ASAP so that sample can be run first thing Monday morning.

 

Off-site Collection: Contact UW Hematopathology at (206) 288-7060 for instructions.

Performing Laboratory

University of Washington Medical Center

Hematopathology Laboratory

G7-800, Seattle Cancer Care Alliance

825 Eastlake Avenue E.

Seattle, WA 98109

 

Phone Number: (206) 606-7060

Department

Department: Send Outs

Phone Number: (206) 987-2563

Methodology

Method: LDT, RT-PCR

Analytical Volume: 6.0 mL Blood OR 1.0 - 2.0 mL Bone Marrow

Limitations:

Reference Range

See individual components.

Send Out Instructions

Reference Test Name: BCR/ABL p190, Quant by RT PCR
Reference Test Number: QP190
Instructions: Send out Monday through Friday with the UW courier.

Special Instructions

Requisition