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Test Code BCR/ABL p210 BCR/ABL p210, Quantitative PCR

Clinical System Name

BCR/ABL p210, Quant

Synonyms

B-ALL

BALL

B-Cell Acute Lymphoblastic Leukemia

BCR-abl detection at FHCRC

Chronic Myelogenous Leukemia

CML

P210

Philadelphia chromosome

QP210

Quantitative BCR/ABL p210

t(9;22)

translocation (9;22)

Description

This test is for monitoring patients who are being treated for chronic myeloid leukemia (CML) and are known to have the p210 (b3:a2 or b2:a2) fusion form. Quantitative p210 BCR/ABL1 testing is performed using the Cepheid GeneXpert system which is able to detect 1 BCR/ABL1 positive cell in 100,000 normal cells. The result is reported as a percent ratio of BCR/ABL1 RNA to ABL1 RNA.

Sample Requirements

Specimen: Whole Blood OR Bone Marrow

Container(s): Lavender/EDTA

Preferred Vol: 10.0 mL Whole Blood OR 2.0 mL Bone Marrow

Minimum Vol: 6.0 mL Whole Blood OR 1.0 mL Bone Marrow

 

Note: Record specimen type and date/time of collection on label.

For more detailed info for bone marrow testing, see separate Bone Marrow Aspirate listing.

Stability

Specimen Type Temperature Time
  Room temp <1 d
  Refrigerated 72 h
  Frozen Unacceptable

Availability

STAT Performed TAT
N M - F 2 w

 

Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temp: 2 - 4 C

Notes: Affix large label to vacutainer.

Storage Location: Store sample in CPA refrigerator Send Outs rack. On weekends, send sample refrigerated to UW NW220 ASAP so that sample can be run first thing Monday morning.

 

Off-site Collection: Contact UW Hematopathology at (206) 288-7060 for instructions.

Performing Laboratory

University of Washington Medical Center

Hematopathology Laboratory

G7-800, Seattle Cancer Care Alliance

825 Eastlake Avenue E.

Seattle, WA 98109

 

Phone Number: (206) 606-7060

Department

Department: Send Outs

Phone Number: (206) 987-2563

 

Methodology

Method: Cepheid GeneXpert Quantitative (Real-Time) Reverse Transcriptase PCR

Analytical Volume: 4.0 mL Whole Blood OR 1.0 mL - 2.0 mL Bone Marrow

Limitations:

Reference Range

See individual components.

Send Out Instructions

Reference Test Name: BCR/ABL p210, Quantitative by RT PCR
Reference Test Number: QP210
Instructions: Send out Monday through Friday with the UW courier.

 

Special Instructions

Requisition