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Test Code BNP B-Type Natriuretic Peptide

Important Note

Specimens drawn in glass tubes will be rejected.

Clinical System Name

B-type Natriuretic Peptide


Brain Natriuretic Peptide


BNP is a neurohormone synthesized primarily by ventricular cardiac myocytes. Increased plasma BNP levels have been observed in adults with congestive heart failure, ventricular hypertrophy, heart transplant rejection, and other conditions. It typically has been used as an aid in the diagnosis and assessment of severity of congestive heart failure, but studies have shown an association between heart transplant rejection and persistent elevated BNP levels, and a reduction in BNP levels with successful treatment of transplant rejection.

Sample Requirements

Specimen:  Whole Blood

Container(s): Lavender/EDTA

Preferred Vol: 1.0 mL

Minimum Vol: 1 FULL Lavender/EDTA Microtainer or 0.5 mL EDTA whole blood


Note: If testing cannot be completed immediately or specimen is collected off site, separate plasma from cells and freeze at -20oC within 7 hours of collection.


Processing Instructions

Reject due to: Incorrect specimen collection, Insufficient quantity of EDTA whole blood drawn, Age of specimen

Spin: N

Aliquot: N

Temp: 2-8oC

Storage location: Core 5 Chemistry Refrigerator


Off-site collection:  Collect in plastic Lavender/EDTA. Spin and aliquot plasma into plastic container within 7 hours of collection, freeze at -20oC. Method affected by prolonged exposure to RBCs.



Specimen Type Temperature Time
EDTA Plasma RT

7 H

EDTA Plasma 2-8oC

 24 H

EDTA Plasma -20oC or -70oC

365 D



Specimen Type Temperature Time
EDTA Whole Blood RT

7 H

EDTA Whole Blood 2-8oC

 7 H





STAT Performed TAT
Y 24/7 1 H


Performing Laboratory

Seattle Children's Laboratory    


Department: Core Chemistry

Phone Number206-987-2617

Reference Range


 Age  pg/mL
0 - 7 D 0 - 170
> 7 D 0 - 41



Method: Fluorescent Immunoassay (FIA) by Triage MeterPro

Analytical Volume: 0.25 mL EDTA Whole Blood or Plasma

CPT Codes