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Test Code BP Bullous Pemphigoid, BP180 and BP230, IgG Antibodies, Serum

Reporting Name

BP 180 and 230, S

Useful For

Bullous pemphigoid (BP) BP180 and BP230 enzyme-linked immunosorbent assay are sensitive, objective, and specific tests that should be considered as an initial screening test in the diagnosis of pemphigoid and its variants.


To compare these results with the standard serum test of indirect immunofluorescence utilizing monkey esophagus substrate.

Specimen Required


Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 1 mL

Specimen Type

Serum Red

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 14 days
  Frozen  30 days
  Ambient  36 hours

Day(s) and Time(s) Performed

Once or twice weekly, Monday through Friday; 7 a.m.- 5 p.m., days of testing to be determined by the laboratory.

Seattle Children's Hospital Note:

TAT: 3 - 7 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Method Description

This enzyme-linked immunosorbent assay (ELISA) method detects and measures serum levels of antibodies of certain pemphigoid diseases. Calibrators and patient sera are added to microwells coated with bullous pemphigoid (BP) BP180 and BP230 antigens, allowing antibodies to react with the immobilized antigens. After washing to remove any unbound serum proteins, horseradish peroxidase-conjugated IgG is added and incubated. Following another wash step, the peroxidase substrate is added and allowed to incubate for an additional period. Stop solution is then added to each well to cancel the enzyme reaction and to stabilize the color development. The assay can be quantified by measuring the reaction photometrically and plotting the results.(Unpublished Mayo method)

Reference Values


<9.0 U (negative)

≥9.0 U (positive)



<9.0 U (negative)

≥9.0 U (positive)

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

83516 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BP BP 180 and 230, S In Process


Result ID Test Result Name Result LOINC Value
88874 BP 180, S 53842-1
28259 BP 230, S 53843-9

Reject Due To


Mild OK; Gross reject


Mild reject; Gross reject


Mild OK; Gross reject