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Test Code BRUCELLA Brucella Antibody Screen, IgG and IgM, Serum

Reporting Name

Brucella Ab Screen, IgG and IgM, S

Useful For

Evaluating patients with suspected brucellosis

Specimen Required


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 1.5 mL

Specimen Type


Specimen Minimum Volume

1.1 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Day(s) and Time(s) Performed

Monday, Wednesday, Friday; 9 a.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Method Description

Serum is tested using an enzyme-linked immunosorbent assay (ELISA) Test Kit containing microtiter strips with wells coated with Brucella abortus antigens (strain W99). In the first reaction step, diluted patient samples are incubated in the wells. Specific IgG or IgM antibodies, if present in the serum, will bind to the antigens. To detect the bound antibodies, a second incubation is carried out using an enzyme-labeled antihuman IgG or antihuman IgM (enzyme conjugate). After addition of the substrate, tetramethylbenzidine (TMB)/hydrogen peroxide and a sulphuric acid stop solution, the resulting color reaction is measured photometrically at a wavelength of 450nm.(Package insert: Anti-Brucella abortus ELISA Test Instruction, Euroimmun Medizinische Labordiagnostika, Lubeck, Germany)

Reference Values


Negative (reported as positive, negative, or equivocal)



Negative (reported as positive, negative, or equivocal)

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86622 x 2-Brucella antibody, IgG and IgM

86622-Brucella total antibody, agglutination (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BRUGM Brucella Ab Screen, IgG and IgM, S In Process


Result ID Test Result Name Result LOINC Value
23685 Brucella Ab Screen, IgG, S 24387-3
23686 Brucella Ab Screen, IgM, S 24388-1
23687 Interpretation 66485-4

Testing Algorithm

If Brucella antibody screen, IgG and IgM is positive or equivocal, then confirmation will be performed at an additional charge.

Reject Due To


Mild OK; Gross reject


Mild OK; Gross reject





Reflex Tests

Test ID Reporting Name Available Separately Always Performed
BRUTA Brucella Ab, Agglutination, S Yes No